Study on the Fluorescence Spectra of LaL3 with DNA
WANG Xing-min1，SONG Yu-min2*，TENG Xiu-lan2, GENG Zhi-yuan2, YANG Pei-ju2, YANG Mei-ling2
1. Basic Sciences Department, Gansu Agriculture University, Lanzou 730070，China 2.Collece of Chemistry and Chemical Engineering, Northwest Normal University, Lanzou 730070，China
Abstract：A fluorescence method is presented for the determination of DNA.The method is based on the interaction of LaL3 [L=morin(2′, 3, 4′, 5, 7-Pentahydroxyflavone), 2′-OH group deprotonated] with DNA in NH3·H2O-NH4Cl buffer of pH=8.0, with λex at 387 nm, and λem at 535 nm. Enhanced fluorescence was observed for LaL3 in the presence of DNA and in the presence of buffer solution of 10%(φ) and ethanol of 10%(φ). The linear range of determination was between 0 and 15 μg·mL-11 for DNA.The method proved to be simple, easy and sensitive for the determination of DNA. The mechanism of enhancing and the reasons for the effects of acid were also discussed.
王兴民1 ，宋玉民2*，滕秀兰2, 耿志远2，杨培菊2，杨美玲2 . 桑色素合镧(Ⅲ)与核酸作用的荧光光谱及其分析应用 [J]. 光谱学与光谱分析, 2004, 24(03): 339-341.
WANG Xing-min1，SONG Yu-min2*，TENG Xiu-lan2, GENG Zhi-yuan2, YANG Pei-ju2, YANG Mei-ling2 . Study on the Fluorescence Spectra of LaL3 with DNA . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(03): 339-341.
