Study on Optical Characteristics of Chromophoric Dissolved Organic Matter (CDOM) In Rainwater by Fluorescence Excitation-Emission Matrix and Absorbance Spectroscopy
CHENG Yuan-yue1，2, GUO Wei-dong2*, LONG Ai-min1, CHEN Shao-yong3
1. LED, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China 2. State Key Laboratory of Marine Environmental Science，College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China 3. Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China
Abstract：The optical characteristics of chromophoric dissolved organic matter (CDOM) were determined in rain samples collected in Xiamen Island, during a rainy season in 2007, using fluorescence excitation-emission matrix spectroscopy associated with UV-Vis absorbance spectra. Results showed that the absorbance spectra of CDOM in rain samples decreased exponentially with wavelength. The absorbance coefficient at 300 nm [a(300)] ranged from 0.27 to 3.45 m-1, which would be used as an index of CDOM abundance, and the mean value was 1.08 m-1. The content of earlier stage of precipitation events was higher than that of later stage of precipitation events, which implied that anthropogenic sources or atmospheric pollution or air mass types were important contributors to CDOM levels in precipitation. EEMs spectra showed 4 types of fluorescence signals (2 humic-like fluorescence peaks and 2 protein-like fluorescence peaks) in rainwater samples, and there were significant positive correlations of peak A with C and peak B with S, showing their same sources or some relationship of the two humic-like substance and the two protein-like substance. The strong positive correlations of the two humic-like fluorescence peaks with a(300), suggested that the chromophores responsible for absorbance might be the same as fluorophores responsible for fluorescence. Results showed that the presence of highly absorbing and fluorescing CDOM in rainwater is of significant importance in atmospheric chemistry and might play a previously unrecognized role in the wavelength dependent spectral attenuation of solar radiation by atmospheric waters.
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