Abstract：The present article studied the interaction between intramolecular charge transfer fluorescence probe-1-keto-2-(p-dimethylaminobenzal)-tetrohydronaphthalene (KDTN) and bovine serum albumins (BSA). With the concentration of KDTN increasing, the fluorescence of BSA rapidly quenched and the fluorescence peak gradually blue-shifted. The result indicated that they were bound mainly by hydrophobic interaction. The binding sites is 0.94 (3 ℃) and the equilibrium constant K is 3.27×104 L·mol-1. Temperature increment is advantageous to the combination. It is a single static quenching process that the fluorescence of BSA quenches, which is induced by the combination of KDTN and BSA. Further study showed that different substances had different effects on the combination of KDTN and BSA.
刘毓芳1,2,李建晴1,2,许志成1,卫艳丽1,双少敏1,董川1*. 一种分子内电荷转移荧光探针与牛血清白蛋白相互作用的研究[J]. 光谱学与光谱分析, 2008, 28(04): 887-890.
LIU Yu-fang1,2,LI Jian-qing1,2,XU Zhi-cheng1,WEI Yan-li1,SHUANG Shao-min1,DONG Chuan1*. Study on the Interaction between ICT Fluorescence Probe and Bovine Serum Albumins. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(04): 887-890.
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