Abstract：The contents of calcium and magnesium in urines were simultaneously determined by flame atomic absorption spectrometry. The optimized working conditions were ascertained. For the determination of calcium, the used wavelength was 422.8 nm, and the current of HCL(Hollow Cathode Lamp) was 3 mA; for the determination of magnesium, the used wavelength was 285.2 nm, and the current of HCL(Hollow Cathode Lamp) was 4 mA. The height of burner and the air-acetylene ratio were 8 mm and 6∶1, respectively, for the determination of both calcium and magnesium. In order to remove the disturbance of phosphate，sulphate and silicate on the determination of calcium, a releasing reagent can be used. Lanthanum chloride (LaCl3) was tested as a better releasing reagent than strontium chloride (SrCl2). The disturbance of urinary substrate coｕld be avoided after the urines were diluted to 1∶100 with distilled water. The concentrations of Ca and Mg in 15 urines were determined under the optimized conditions. The obtained results were consistent with the archived data. The recovery was 96%-104%, the relative standard deviation for a sample was 1.8% with P＜0.05.
[1] OUYANG Jian-ming，YAO Xiu-qiong，SU Ze-xuan et al(欧阳健明，姚秀琼，苏泽轩等). Science in China(中国科学)，B辑，2003，33(1)：14. [2] OUYANG Jian-ming，YAO Xiu-qiong，ZHONG Jiu-ping et al(欧阳健明，姚秀琼，钟玖平等). Chemical Journal of Chinese Universities(高等学校化学学报)，2002, 23(12)：2237. [3] OUYANG Jian-ming(欧阳健明). Chinese Chemistrｙ Bｕlletin(化学通报), 2002, (5): 326. [4] CAO Lü-cheng, ZHANG Shao-shun(曹履诚，章绍舜). The Foundament of Urostealith and Clinical Research(尿石症基础与临床研究). Shandong: Shandong Science and Technology Press(山东：山东科技出版社)，1990. 280. [5] SUN Jing-zhang(孙景章). Hebei Pottery(河北陶瓷)，1997，25(4)：15. [6] HAO Ai-guo，YANG Bo，HAN Ying(郝爱国，杨 波，韩 颖). Chinese Journal of Spectroscopy Laboratory(光谱实验室)，1999，16(3)：278. [7] YAO Jun，MA Ming-you，CHEN Shang et al(姚 俊，麻明友，陈 上等). Journal of Jishoｕ University(吉首大学学报)(Natural Science Edition) (自然科学版)，2000，21(1)：50. [8] MA Ge, ZHANG Jing-yan(马 戈，张景彦). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，1999，19(4)：627. [9] LI Sheng-liang(李盛亮主编). The Spectral Method of Atomic Absorption(原子吸收光谱法). Shanghai: Shanghai Science and Technology Press(上海：科学技术出版社)，1989. 133. [10] LI Zhi-ｇanｇ，ZHAN Binｇ-ｙan，DONG Ｗen-ｋｕi(李智刚，詹炳炎，董文奎). Joｕrnal of Clinical Sｕrｇerｙ(临床外科杂志)，1999，7(2)：85.
