A Comparative Study of Malignant Tissue Diagnosis Using ATR and Microscopy FTIR Spectroscopy
REN Yu1,2, XU Yi-zhuang1, ZHAO Ying3, YANG Li-min1, LI Qing-bo1, ZHANG Yuan-fu1, WENG Shi-fu1, SHI Jing-sen2, XU Duan-fu3, WU Jin-guang1*
1. The State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 2. First Hospital of Xi'an Jiaotong University, Xi'an 710061, China 3. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China
Abstract：A comparative FTIR study was undertaken between ATR (attenuated total reflection) and infrared microscopy on their application to measuring the same tissue for cancer diagnosis. The measurements showed that the coincidence of pathological diagnosis with ATR is higher than that of microscopy FTIR method. The result indicated that the measured area of tumor tissue is critical. The area for microscopy measurement is ～250 μm2, it reflects the heterogeneity of the tissue. However, the ATR measurement provides more information of malignant tissue for the measured area is 1.9×105 times than microscopy. Therefore, ATR exhibit the high accuracy in cancer detection for clinical application.
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