1. Key Laboratory of Education Ministry for Medicinal Chemistry of Natural Resource, College of Chemical Science and Technology, Yunnan University, Kunming 650091, China 2. Zhongguancun Middle School of Beijing City at Haidian District, Beijing 100190, China 3. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Abstract：Elemental compositions in spider and silkworm silks were determined by X-ray fluorescence (XRF) spectrum to probe the silk-forming mechanisms and an elemental basis for spider silk with excellent characteristics. XRF analysis demonstrates that in the silkworm silk, the elemental content is 47.10% for C, 29.92% for O and 16.52% for N, including metal elemental contents: 0.166 2% for Ca, 0.104 0% for Mg and 0.039 5% for K, while Na, Zn, Ni, Fe and Cr show less micro quantity. Due to relative high quantity for Ca and Mg, they both play an important role in the silk-forming mechanism by silkworm. In the spider silk, the determined main nonmetal elemental contents are 44.09% for C, 26.64% for O and 22.34% for N. The high content of nitrogen may be an elemental basis for spider silk with excellent characteristic. The main metal elemental contents are 0.268 0% for Na, 0.081 4% for K and 0.011 6% for Mg, while Ca, Zn, Ni, Cu and Cr possess less micro quantity in the spider silk. Because of relative high quantity for Na and K, they both play an important role in the silk-forming mechanism by spider. The elemental compositions investigated by using mathematic statistic method are quite in agreement with those demonstrated by using XRF spectrum, which validates the experimentally determined elemental compositions in the spider and silkworm silks.
[1] Randolph V L. Chem. Rev., 2006, 106: 3762. [2] Simmons A H, Michal C A, Jelinski L W. Science, 1996, 271: 84. [3] Vollrath F, Knight D P. Nature, 2001, 410: 541. [4] Shao Z Z, Vollrath F. Nature, 2002, 418: 741. [5] Gosline J M, Denny M W, DeMont M E. Nature, 1984, 309: 551. [6] Xu M, Lewis R V. PNAS(Proceedings of the National Academy of Science of the States of America), 1990, 87: 7120. [7] PAN Zhi-juan, LI Chun-ping, QIU Xin-wei(潘志娟, 李春萍, 邱芯薇). Science Technology and Engineer(科学与技术工程), 2002, 2: 30. [8] Yang Z, Liivak O, Seidel A, et al. J. Am. Chem. Soc., 2000, 122: 9019. [9] HUANG Jun-ting(黄君霆). Bulletin of Sericulture(蚕桑通报), 2001, 32: 1. [10] CHEN Hua, ZHU Liang-jun, MIN Si-jia(陈 华, 朱良均, 闵思佳). Bulletin of Science and Technology(科技通报), 2003, 19: 260. [11] ZHOU Li, TERRY A E, HUANG Yu-fang, et al(周 丽, TERRY A E, 黄郁芳, 等). Acta Chimica Sinica(化学学报), 2005, 63: 1379. [12] CHEN Xin, HUANG Yu-fang, SHAO Zheng-zhong, et al(陈 新, 黄郁芳, 邵正中, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2004, 25: 1160. [13] ZHOU Wen, HUANG Yu-fang, SHAO Zheng-zhong, et al(周 文, 黄郁芳, 邵正中, 等). Acta Chimica Sinica(化学学报), 2007, 65: 2197. [14] Kaplan D, Wada A. ACS Symposium Series 544. New York：ACS Press，1994. [15] Gui Z Z, Zhuang D H. Canye Kexue, 2002, 28：301. [16] Zhu L J, Yao J M, Li Y L. J. Zhejiang Agricultural University, 1998, 24: 268. [17] Lombardi S J, Kaplan D L. J. Arachnol., 1990, 18: 297. [18] Anderson S O. Comparative Biochem. Physiol., 1970, 35: 705. [19] Chen X, Knight D P, Vollrath F. Biomacromolecules, 2002, 3: 644. [20] CHEN Xin, SHAO Zheng-zhong，Knight D P, et al(陈 新，邵正中，Knight D P, 等). Acta Chimica Sinica(化学学报), 2002, 60: 2203. [21] LI Gui-yang, ZHOU Ping, SUN Yao-jun, et al(李贵阳, 周 平, 孙尧俊, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2001, 22: 860. [22] ZONG Xiao-hong, ZHOU Ping, SHAO Zheng-zhong, et al(宗小红，周 平，邵正中，等). Chinese Science Bulletin(科学通报)，2005, 50: 1080. [23] Zhou P, Xie X, Knight D P, et al. Biochemistry, 2004, 43: 11302.
