强荧光背景环境中荧光涨落谱——蒙特卡罗模拟研究

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摘要：荧光涨落谱方法(Fluorescence Fluctuation Spectroscopy)通过分析微小探测区域内的荧光涨落信号，获得粒子亮度、扩散系数以及溶液浓度等信息。利用Monte Carlo模拟方法，研究了溶液中自荧光背景和系统噪声对荧光涨落谱的影响。结果表明，利用双组分光子计数统计方法，可以有效去除低亮度、高浓度背景组分自荧光和均匀分布系统噪声产生的影响。本结果为利用荧光涨落谱方法测量细胞体系复杂环境中的蛋白质相互作用提供帮助。

关键词：光子计数统计;荧光涨落谱;Monte Carlo模拟

Abstract：Fluorescence fluctuation spectroscopy is a method in which fluorescence fluctuations arising from a very small sample volume are analysed to obtain brightness, diffusion coefficient and concentration of particles. Using Monte Carlo simulation, the influences of background autofluorescence and noises on fluorescence fluctuation spectroscopy are studied. Results show that a two- component photon counting histogram can effectively remove the effects due to background autofluorescence from low brightness and high concentration components and uniform noise. The result will help to bring applications of fluorescence fluctuation spectroscopy to intracellular environment.

Key words：Photon counting histogram;Fluorescent fluctuation spectroscopy;Monte Carlo simulation

收稿日期: 2003-02-08 修订日期: 2003-04-28

中图分类号:	O56
	Q6

通讯作者: 马辉

引用本文:

刘广，陈波，孟凡波，马辉^*，陈瓞延 . 强荧光背景环境中荧光涨落谱——蒙特卡罗模拟研究[J]. 光谱学与光谱分析, 2004, 24(11): 1379-1383.
LIU Guang, CHEN Bo, MENG Fan-bo, MA Hui^*, CHEN Die-yan . Fluorescence Fluctuation Spectroscopy with Strong Background Fluorescence——An Monte Carlo Approach. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1379-1383.

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http://www.gpxygpfx.com/CN/Y2004/V24/I11/1379