Abstract：In this paper，the medium (pH，organic solvent and surfactant) effects on fluorescence of eosin-5-isothiocynate(eosin-ITC) have been studied in detail. The results show that the pH and polarity of system studied are two great important influence factors on fluorescence of eosin-ITC. The fluorescence intensity of eosin-ITC in weak acidic medium is higher obviously than that of in weak basic medium， but it is sensitive to change of pH， be careful to use. The reaction between surfactant and eosin-ITC shows a good electric selectivety，and the effect of CTMAB on fluorescence of eosin-ITC can be classified as two stages: monomer quenching (below cmc) and micelle sensibilization (above cmc). In the presence of micelle of CTMAB， the fluorescence intensity of eosin-ITC in weak basic medium is even higher than that of in weak acidic medium. In the presence of high organic solvent content，the fluorescence intensity of eosin-ITC is also increased obviously. These properties are available for use.
吴娅兰1，李隆弟1*，刘佳铭2，朱国辉2 . 发光标记物——异硫氰酸曙红荧光的介质效应[J]. 光谱学与光谱分析, 2004, 24(12): 1597-1600.
WU Ya-lan1，LI Long-di1*，LIU Jia-ming2，ZHU Guo-hui2 . The Medium Effects of Fluorescence of Eosin-5-Isothiocynate as a Luminescent Marker . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(12): 1597-1600.
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