FTIR Study on the Finger Nail with Carcinoma of Nasopharynx
DONG Qin1, LIU Gang2,LIU Tian-hui3
1. Center of Experiment, Yunnan University, Kunming 650091, China 2. Center of Analysis and Testing, Yunnan Normal University, Kunming 650092, China 3. First Hospital of Yunnan Province, Kunming 650000, China
Abstract：Finger nails from twenty normal people and three with carcinoma of nasopharynx were studied by Fourier Transform Infrared (FTIR) technique. The results showed that there are obvious differences between FTIR spectra of them in spectral parameters such as frequency, intensity and band shape etc. The most striking differences in the spectra were observed in the change of amide Ⅱ, the disappearance of δｓ(CH3) peak, and at 874.0 cm-1 whether appeared an absorption peak. The changes involving the phosphate symmetric stretching νs, PO-2 and asynmmetric stretching νas, PO-2 modes, the CH3 and CH2 groups stretching (νs, CH2, νas, CH3) bending (δ (CH3)) modes and the C—O stretching ν (C—O) mode were discussed. In addition, the changes of structure of hydrogen-bonding of nucleic acid and cell proteins and the packing and the conformational structure of the membrane lipids were analysed. The average wave number of band of νs (PO-2) shifted from 1 080.0 to 1 077.6 cm-1 and that of νas (PO-2) shifted from 1 239.4 to 1 238.4 cm-1, which indicated that the degree of hydrogen-bonding formed by oxygen atom of the phosphodiester groups of nucleic acids was weakened. The average wave number of band of δ (CH2) of membrane lipids shifted from 1 453.1 to 1 453.7 cm-1, and its peak intensity was slightly enhanced, which suggested that the conformational structure of the methylene chains of membrane lipids is more disordered than in normal nail.
董 勤1，刘 刚2，刘天惠3 . 鼻咽癌患者指甲的红外光谱研究[J]. 光谱学与光谱分析, 2004, 24(12): 1543-1545.
DONG Qin1, LIU Gang2,LIU Tian-hui3 . FTIR Study on the Finger Nail with Carcinoma of Nasopharynx. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(12): 1543-1545.
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