Analysis of Soil Humus and Components after 26 Years’ Fertilization by Infrared Spectroscopy Method
ZHANG Yu-lan1, 3, SUN Cai-xia2, CHEN Zhen-hua1, LI Dong-po1, LIU Xing-bin1, CHEN Li-jun1*, WU Zhi-jie1, DU Jian-xiong2
1. Group of Applied Soil and Biochemistry, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China 2. College of Science, Northeastern University, Shenyang 110004, China 3. Cold and Arid Regions Engineering and Environment Research Institute, Chinese Academy of Sciences, Lanzhou 730009, China
Abstract：The infrared spectrum was used to discuss structure change of soil humus and components of chemical groups in soil humic acids (HA) and fulvic acids (FA) isolated from soils in different fertilization treatment after 26 year’s fertilization. The result indicated that using the infrared spectroscopy method for the determination of humus, humus fractions (HA and FA) and their structure is feasible. Fertilization affected the structure and content of soil humus and aromatization degree. After 26 years’ fertilization, the infrared spectrum shapes with different treatments are similar, but the characteristic peak intensity is obviously different, which reflects the effects of different fertilization treatments on the structure and amounts of soil humus or functional groups. Compared with no fertilization, little molecule saccharides decreased and aryl-groups increased under application of inorganic fertilizer or combined application of organic and chemical fertilizer. The effect was greater in Treatment NPK and M+NPK than in Treatment M1N and M2N. Organic and NPK fertilizer increased the development of soil and increased soil quality to a certain extent. Results showed that organic fertilization increased aromatization degree of soil humus and humus fractions distinctly. The authors could estimate soil humus evolvement of different fertilization with infrared spectroscopy.
Key words：Brown soil;Long-term located fertilizer experiments;Humus;Structure;Far infrared spectroscopy
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