1. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China 2. College of Science, Nanjing Agricultural University, Nanjing 210095, China 3. Institute of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
Abstract：Fourier transform infrared spectroscopy (FTIR), ultraviolet spectroscopy (UV)，and nuclear magnetic resonance spectroscopy (NMR) were used to study the chemical composition of Dissolved Organic Matter (DOM) derived from rice straw in the hydrolysis process with a dilute complex acid solution. The results obtained are as follows. FTIR spectra could indicate the changes of DOM during the hydrolysis process of rice straw. With the progress of rice straw hydrolysis, methyl, methylene, aromatic compounds and carbonhydrates decreased，most of aliphatic compounds were oxidized to CO2 and H2O, and others were turned into carbonates. Most of the organic silicon was hydrolyzed into inorganic silicon. The proteins, amino acids and other nitrogen were hydrolyzed to NH+4. All the recalcitrant fractions of rice straw, such as hemi-cellulose, cellulose and silicon sharply decreased during the process of chemical treatment. The results obtained in this paper proposed that the changes of DOM of rice straw in the hydrolysis could be an indication in the changes of chemical composition of rice straw during the hydrolyzation，and FTIR, UV and NMR were good methods to study the changes in the structure of organic compounds.
[1] XU Yong, SHEN Qi-rong, ZHONG Zeng-tao, CHEN Xiang-huai(徐 勇，沈其荣，钟增涛，陈湘淮). Scientia Agricultura Sinica(中国农业科学), 2003，36(1): 59. [2] ZHANG Ya-li, ZHANG Juan, WANG Jin-chuan, SHEN Qi-rong(张亚丽，张 娟，王金川，沈其荣). Chinese Journal of Applied Ecology(应用生态学报)，2002，13(12)：1575. [3] GU Xun-gang, WANG Guo, FANG Ling(谷勋刚，王 果，方 玲). Plant Nutrition and Fertilizer Science(植物营养与肥料学报)，2001，7(1)：93. [4] SHEN Qi-rong, YIN Shi-xue, YANG Chao-guang, CHEN Wei(沈其荣，殷士学，杨超光，陈 魏). Plant Nutrition and Fertilizer Science(植物营养与肥料学报)，2000，6(1)：98. [5] ZHAN Xin-hua, ZHOU Li-xiang, SHEN Qi-rong, HUANG Huan-zhong(占新华，周立祥，沈其荣，黄焕忠). Acta Scientiae Circumstantiae(环境科学学报)，2001，21(4)：470. [6] WU Jing-gui, XI Shi-quan, ZENG Guang-bin, WANG Dong-mei, JIANG Yan, WANG Ming-hui(吴景贵，席时权，曾广斌，汪冬梅，姜 岩，王明辉). Acta Pedologica Sinica(土壤学报)，1999，2 (1)：91. [7] WU Jing-gui, XI Shi-quan, ZENG Guang-bin, WANG Dong-mei, JIANG Yan, WANG Ming-hui(吴景贵，席时权，曾广斌，汪冬梅，姜 岩，王明辉). Short Communication of Analytical Chemistry(分析化学研究简报)，1998，26(11)：1380. [8] Kuwatsuka S, Watanabe A, Itoh K, Aria S. Soil Sci. Plant Nutr., 1992, 38(1): 23. [9] Inbar Y, Ｃhen Y, Hadar Y. Soil Sci., 1991, (4): 272. [10] During D T, Esterle J S, Dickson T J, During J R. Appl. Spectosc., 1988, 42: 1239. [11] Inbar Y, Chen Y, Hadar Y. Soil Sci. Am. J., 1989, 53: 1695. [12] Koji Nakanshi, Solomon P H. 100 Cases of Infrared Spectrum Analysis(红外光谱分析100例). Beijing: Science Press(北京：科学出版社)，1984. ８. [13] WU Jing-gui, ZENG Guang-bin, WANG Dong-mei, XI Shi-quan, JIANG Yan, WANG Ming-hui, GENG Yu-hui(吴景贵，曾广斌，汪冬梅，席时权，姜 岩，王明辉，耿玉晖). Short Communication of Analytical Chemistry(分析化学研究简报)，1997，25(12)：1395. [14] WU Jing-gui, JING Feng-ying, WANG Dong-mei, XI Shi-quan, JIANG Yan, WANG Ming-hui, JIANG Yi-mei, GENG Yu-hui(吴景贵，景凤英，汪冬梅，席时权，姜 岩，王明辉，姜亦梅，耿玉晖). Short Communication of Analytical Chemistry(分析化学研究简报)，1999，27(8)：933. [15] Preston Caroline M, Trofymow J A (Tony), Sayer Brian G, Junning Niu. Can. J. Bot.，1997, 75: 1601. [16] Almendros G, Dorado J, González-Vila F J, Blanco M J, Lankes U. Soil Biology & Biochemistry，2000, 32: 793. [17] Wershaw R L. Humic Substances in Soils, Sediment and Water. In Ajken G R et al.(ed.). New York：Wiley-Intersci., 1985. 561 [18] Knicker H, Udemann H D L，Haider K. European Journal of Soil Science, 1997, 48：431.
