Differentiation of Rhododendron Based on the Infrared Spectra of Petals
LUO Bi-rong1, LIU Gang1*, ZHANG Yu-bin1, ZHOU Xiang-ping1, SANG Lin2
1. Department of Physics and Electron Information, Yunnan Normal University, Kunming 650092, China 2. Department of Life Sciences, Yunnan Normal University, Kunming 650092, China
Abstract:Several techniques were used to identify and classify plants. Mid-infrared spectroscopy combined with appropriate software was used in an attempt to differentiate different subgenus from Rhododendron. Fourier transform infrared (FTIR) spectroscopy was used for obtaining vibrational spectra of 46 petals from Rhododendron. Very minor differences were observed in the FTIR spectra among four subgenuses. For the purpose of rapid differentiation, libraries of spectra were created using samples from each subgenus variety. Spectra of unknown samples were recorded and compared with those of the libraries and the rate of affinity (the match value) was measured automatically using the appropriate software (OMNIC). The results showed that petal samples from different subgenus varieties can be differentiated from each other. The study demonstrates that combining FTIR spectroscopy with appropriate analysis method can classify Rhododendron plants at subgenus level. It offers a potential method for the taxonomic research on plants system.
罗庇荣1,刘 刚1*,张玉宾1,周湘萍1,桑 林2 . 基于花瓣的傅里叶变换红外光谱鉴别分类杜鹃属植物 [J]. 光谱学与光谱分析, 2010, 30(04): 943-948.
LUO Bi-rong1, LIU Gang1*, ZHANG Yu-bin1, ZHOU Xiang-ping1, SANG Lin2 . Differentiation of Rhododendron Based on the Infrared Spectra of Petals . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(04): 943-948.
[1] FANG Rui-zheng(方瑞征). Flora Reipublicae Popularis Cinicae(中国植物志). Beijing: Science Press(北京: 科学出版社), 1999. [2] CHEN Xun(陈 训). China Journal of Chinese Materia Medica(中国中药杂志), 1999, 24(6): 334. [3] Gary C H, Valerie A, Margi C, et al. Plant Science, 2006, 170: 314. [4] Mubo A S, Adeniyi A J, Adeyemi E. Biochemical Systematics and Ecology, 2005, 33: 79. [5] ZHAO Xi-hua, ZHANG Le-hua, WANG Man-ying, et al(赵喜华, 张乐华, 王曼莹, 等). Acta Agriculturae Universitatis Jiangxiensis(江西农业大学学报), 2006, 28(4): 544. [6] Lamprell H, Mazerolles G, Kodjo A, et al. International Journal of Food Microbiology, 2006, 108: 125. [7] Klinkenberg M, Dohrmann R, Kaufhold S, et al. Applied Clay Science, 2006, 33: 195. [8] Lü Hongfei, CHENG Cungui, TAN Xi, et al. Acta Botanica Sinica, 2004, 46(4): 401. [9] Ritsuko H, Junji S. Carbohydrate Polymers, 2003, 52: 449. [10] Mathilde F, Herman H, Samantha V. Plant Physiol. Biochem., 2000, 38(1/2): 15. [11] LUO Bi-rong, LIU Gang, SHI You-ming, et al(罗庇荣, 刘 刚, 时有明, 等). Infrared Technology(红外技术), 2009, 31(1): 39. [12] Tarantilisa P A, Troianou V E, Pappas C S, et al. Food Chemistry, 2008, 111: 192. [13] CAO Ming-ju, ZHENG Xiao-yan, et al(曹明菊, 郑晓燕, 等). South China Agriculture(南方农业), 2007, 4(8): 56. [14] RUAN Hai-xing, WANG Zi-jian, WU Ke-feng, et al(阮海星, 王子坚, 吴克枫, 等). Journal of Plant Resources and Environment(植物资源与环境), 1997, 6(1): 25. [15] ZHANG Qi-han, GUO Jian, ZHANG Ming-zhe, et al(张奇涵, 郭 健, 张明哲, 等). Acta Scientiarum Naturalium Universitatis Pekinensis(北京大学学报), 1996, 32(6): 703. [16] XU Mei-yi, HAN Ya-li, DONGYE Guang-zhi, et al(徐美奕, 韩雅莉, 东野广智, 等). Journal of Chinese Medicinal Materials(中药材), 2007, 30(10): 1252. [17] YANG Guan-yu, ZHANG Jing-hua, ZOU Wei-hua, et al(杨贯羽, 张敬华, 邹卫华, 等). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2006, 23(2): 390. [18] Sevgi T G, Musa D, Feride S. Applied Spectroscopy, 2007, 61: 300. [19] XIE Jing-xi, CHANG Jun-biao, WANG Xu-ming(谢晶曦, 常俊标, 王绪明). Application of Infrared Spectroscopy in Organic Chemistry and Pharmaceutical Chemistry(红外光谱在有机化学和药物化学中的应用). Beijing: Science Press(北京: 科学出版社), 2001. 77.