Abstract：A simple, rapid and inexpensive fluorimetric method for simultaneously determining ASA and SA in a single scan of the co-existing specimen had been established. When the wavelength interval(Δλ) is 80 nm for scaning, the amounts of ASA and AS could be quantitatively determined respectively. The fluorescence intensity was linearly related to the aspirin concentration in the range of 4.0×10-6-1.0×10-4 mol·L-1 with correlation coefficient 0.994 9; the fluorescence intensity was linearly related to the salicyclic acid concentration in the range of 8.0×10-7-1.0×10-4 mol·L-1 with correlation coefficient 0.997 5, the determination limit was 4.0×10-7 mol·L-1. The method is simple, rapid and inexpensive, it can be used for medicament analysis.
[1] USP 23, The United States Pharmacopceial Convention Inc., Rockville, M D, 1994. 136. [2] British Pharmacopoeia, London：Her Majesty's Stationery Office, 1９９８. 901. [3] WU Zhou-an，WU Sheng-qing(吴周安, 吴盛清). Chin. J. Anal. Chem.(分析化学)，1997，25(8)：991. [4] Gillian P M, Shane J Q, Desmond J F et al. J. Chromatogr. B, 1998，707(1～2): 322. [5] Pirola R, Bareggi S R, Benedittis G D. J. Chromatogr. B, 1998, 705(13): 309. [6] Zenon K, Kinga B. J. Pharm. Biomed. Anal., 1998, 18: 871. [7] China Pharmacopoeia(中国药典, 二部). 2000. Vol Ⅱ：328. [8] Miles C I, Schenk G H. Anal. Chem., 1970, 42(6): 656. [9] Shane N A, Stillman R. J. Pharm. Sci., 1971, 60(1): 114 (Eng). [10] HE Wen-qi, YAO Wei-xi, XIE Xue-peng(何文琪，姚渭溪，谢学鹏). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，1996，16(4)：100.
