FTIR Spectra-Principal Component Analysis of Phenetic Relationships of Huperzia Serrata and Its Closely Related Species
GUO Shui-liang1，2, LI Pei-ling1,FANG Fang1,HUANG Hua1,CHENG Cun-gui1
1. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China 2. College of Life and Environmental Sciences, Shanghai Teachers' University, Shanghai 200234, China
Abstract：Huperzia serrata is an important medicinal plant. This species is rich in inner-specific variation with various closely related species, and their individuals are also small with few identification characters. In the present paper, the method of Fourier transform infrared spectrometer with an OMNI collector was applied to obtaining the infrared spectra of 16 leaf samples including Huperzia serrata and its five closely related species (Huperzia sutchueniana, Phlegmariurus mingchegensis, Lycopodium japonicum, Selaginella doederleinii, Selaginella heterostachys). Based on the indices of wave number-absorbance, the differences of the 16 infrared spectra were compared by the method of Principal Component Analysis (PCA). The results showed that there is good correspondence between the position relationship of PCA three-dimensional plot of the samples based on the indices of wave number-absorbance of FTIR spectra and their phenetic relationship. Therefore, the infrared spectra could be applied to identifying the samples of Huperzia serrata and its closely related species.
郭水良1，2，李沛玲1，方 芳1，黄 华1，程存归1 . 蛇足石杉及其近缘种表征关系的FTIR主成分分析[J]. 光谱学与光谱分析, 2005, 25(05): 693-697.
GUO Shui-liang1，2, LI Pei-ling1,FANG Fang1,HUANG Hua1,CHENG Cun-gui1 . FTIR Spectra-Principal Component Analysis of Phenetic Relationships of Huperzia Serrata and Its Closely Related Species . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(05): 693-697.
