Determination of Dopamine Hydrochloride by a Reverse Flow Injection Chemiluminescence Method
WANG Shu-hao1, 2,DU Ling-yun1,WEI Xin-ting1,WANG Liang-yu1,ZHUANG Hui-sheng2
1. Institute of Chemistry and Chemical Technology，Liaocheng University，Liaocheng 252059, China 2. College of Environmental Science and Engineering, Donghua University, Shanghai 200051, China
Abstract：A novel chemiluminescence system coupled with a reverse flow injection analysis for the determination of dopamine hydrochloride was presented. It is based on the strong quench effect of dopamine hydrochloride on the chemiluminescence reaction between luminol and hexacyanoferrate(Ⅲ) under alkaline condition. Various factors affecting the chemiluminescence intensity of the system were investigated. The possible mechanism of the proposed method was also studied. The decrease of chemiluminescence intensity was linear with the dopamine hydrochloride content in the range of 2.0×10-9-8.0×10-7 g·mL-1, the detection limit of the method was 1.14×10-9 g·mL-1, and the relative standard deviation was 0.99%(4.0×10-7 g·mL-1, n=11). It was successfully used for the determination of the content of dopamine hydrochloride in dopamine hydrochloride injection.
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