高效液相色谱化学发光测定中药制剂中2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷

摘要
参考文献
相关文章 (15)

全文: PDF (476 KB)
输出: BibTeX | EndNote (RIS)

摘要：研究了2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在硝酸介质中与高锰酸钾的发光行为和光谱现象，甲醛的存在可使化学发光强度大大增强。由于中药制剂成分复杂，干扰大，文章首次采用反相高效液相色谱化学发光技术，建立了一种测定中药制剂中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的新方法。对于不同中药制剂样品中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷测定的回收率为102%～108%。方法的检出限为 11.83 μg·mL^-1，线性范围为15.75～136.5 μg·mL^-1，相对标准偏差为3.45 %(Cs=21.0 μg·mL^-1，n=3)。此法简便、快速，重复性好，结果令人满意。

关键词：高效液相色谱;化学发光分析;高锰酸钾;2;3;5;4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷

Abstract：Based on the chemiluminescence reaction of KMnO₄-HNO₃-2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside sensitized by HCHO, an analytical method for 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside by RP-HPLC with chemiluminescence detector was developed. The chromatographic conditions were optimized. AN ODS hypersil column(25 mm×4.0 mm, 125×4.0 mm i.d. 5 μm) was used with acetonitrile-0.3% H₃PO₄[25∶75, (φ)] as mobile phase, and the flow rate was 0.8 mL·min^-1 at the column temperature of 40 ℃. The linear range of calibration curve was from 15.75 to 136.5 μg·mL^-1, the detection limit was 11.83 μg·mL^-1 with a concentration of 21 μg·mL^-1 of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, and the recovery was 102%-108%. The relative standard deviation was 3.45%(Cs=21.0 μg·mL^-1, n=3). The method has been applied to the determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in a few Chinese medicines, and the results were satisfactory. The method was simple, rapid and highly selective.

Key words：HPLC;Chemiluminescence;Potassium permanganate;2;3;5;4′-tetrahydroxystilbene-2-O-β-D-glucoside

收稿日期: 2003-12-16 修订日期: 2004-03-28

中图分类号:

O657.3

通讯作者: 刘二保

引用本文:

卫洪清^1，2，刘二保^1*，任荣芳¹，赵秀丽²，李晓霞²，江凤仙² . 高效液相色谱化学发光测定中药制剂中2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷[J]. 光谱学与光谱分析, 2005, 25(06): 844-847.
WEI Hong-qing^1，2, LIU Er-bao^1*, REN Rong-fang¹, ZHAO Xiu-li²，LI Xiao-xia², JIANG Feng-xian² . Determination of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside in the Traditional Chinese Preparation by High Performance Liquid Chromatography with Chemiluminescence Detection . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(06): 844-847.

链接本文:

https://www.gpxygpfx.com/CN/Y2005/V25/I06/844

[1] LU Yan-wei(卢兖伟). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(4): 759.
[2] JIANG Cong-qiu, GAO Ming-xia(江崇球, 高明霞). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(5): 937.
[3] XU Yong-qun, SUN Su-qin, FENG Xue-feng，HU Shi-lin(徐永群, 孙素琴, 冯学峰，胡世林). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(3): 502.
[4] WANG Zhen-gang, et al. Trends in Pharmacological Sciences, 2002, 23(8): 347.
[5] Ku Yoe-ray, et al. J. Chromatography A, 1999, 848: 537.
[6] Lau Aik-Jiang. J. Pharmaceutical and Biomedical Analysis, 2003, 31(2): 401.
[7] DENG Wen-long, et al(邓文龙, 等). J. Chinese Herbal Medicine(中草药), 1987, 18(3): 42.
[8] MEI Mei-zhen, et al(梅美珍, 等). Acta Pharmaceutica Sinica(药学学报), 1979, 14(1): 8.
[9] SU Wei, et al(苏玮, 等). J. Chinese Herbal Medicine(中草药), 1997, 28(2): 119.
[10] Pharmacopoeia Committe of Ministry of Public Health, P.R.China(中华人民共和国卫生部药典委员会编). The Pharmacopoeia of People's Republic of China(Part 1)(中华人民共和国药典·一部). Beijing: Chemical Industry Press(北京：化学工业出版社), 2000. 139.
[11] MA Chang-hua, et al(马长华, 等). Chinese Traditional Medicine(中药材), 1995, 18(7): 662.
[12] YAO Gui-gen, et al(姚桂根, 等). Chinese J. Pharmaceutical Anal.(药物分析杂志), 1984, 4(1): 28.
[13] YAO Gui-gen, et al(姚桂根, 等). Chinese Traditional Patent Medicine Study(中成药研究), 1986, (10): 10.
[14] LI Guang-dao, et al(李光道, 等). J. Tongji Medical Courses in General University(同济医科大学学报), 1995, 24(4): 289.
[15] YUAN Hai-long, et al(袁海龙, 等). Pharm. J. Chin. PLA.(解放军药学学报), 2000, 16(3): 152.
[16] WANG Chun-ying, et al(王春英, 等). Acta Pharmaceutical Sinica(药学学报), 2002, 37(12): 955.
[17] LU Jing，et al(鲁静，等). Research on Shizhen Medicine of the Nation(时珍国医国药), 2001, 12(9): 104.