Spectrum Analysis of Erythrocyte Intracellular Hemoglobin with a Novel Fast Multi-Channel Micro-Spectrophotometry
RUAN Ping1, HUANG Yao-xiong2, LI Dan3
1.Department of Physics, Guilin Medical College, Guilin 541004, China 2.Institute of Biomedical Engineering, Jinan University, Guangzhou 510632, China 3.College of Physics and Informational Technology, Guangxi Normal University, Guilin 541004, China
Abstract：With the application of a novel fast multi-channel micro-spectrophotometry(MMSP), the absorption spectrum of intracellular hemoglobin molecule in a human single living red blood cell was monitored under the altered physiological environments (pH, temperature, partial pressure of oxygen, and osmotic pressure).It was found that on the absorption spectrum curve of hemoglobin the spike, the height and the position of the characteristic absorption peaks at 540 and 575 nm were altered obviously with the changes in physiological conditions, indicating that the concentration, structure and function of the hemoglobin in the red blood cell may be closely associated with the physiological environments.
Key words：Novel fast multi-channel micro-spetrophotometry;Hemoglobin;Absorption spectrum;Partial pressure of oxygen;Osmotic pressure
[1] John E, William J, John W.Journal of Microscopy, 1984, 133(3)： 313. [2] Kohen E, Gatt S, Schachtschabel A, et al.Biotechnol.Appl.Biochem., 1999, 29(3): 191. [3] Kavanagh K Y, Murphy E J, Harmey M, et al.Physiol.Meas., 1999, 20(3): 303. [4] Meyer B, Schaller C, Frenkel C, et al.Stroke, 1999, 30(12): 2623. [5] ZENG Yi-tao(曾溢滔主编).Human Ｈemoglobin(人类血红蛋白).Beijing: Science Press(北京: 科学出版社), 2002. [6] QIN Ren-jia(秦任甲主编).Hemorheology(血液流变学).Beijing: People's Medical Publishing House(北京: 人民卫生出版社), 1999. [7] RUAN Ping, HUANG Yao-xiong, TU Mei, et al(阮 萍, 黄耀熊, 屠 美, 等).J.Fourth Millitary Medical University(第四军医大学学报), 2003, 24(23): 2119. [8] LI Jing, et al(李 晶，等).Acta Biophysica Sinica(生物物理学报), 2002, 18: 350. [9] Kazuhiro T, Takuo S, Katsuko K.J.Biochem., 1997, 122: 264. [10] YAO Cheng-can, HUANG Yao-xiong, RUAN Ping, et al(姚成灿, 黄耀熊, 阮 萍，等).Chinese Science Bulletin(科学通报), 2003, 48(10): 1342. [11] JI Tao(籍 涛).Micro-Spectrophotometry and Image Analysis with Applications in Structure and Function Simultaneous Measurement on Single Intact Cell.Guangzhou: Sun Yat-sen University of Medical Sciences(广州: 中山医科大学), 2001. [12] TIAN Feng-hua, DING Hai-shu, WANG Guang-zhi, CAI Zhi-gang, ZHAO Fu-yun(田丰华, 丁海曙, 王广志, 蔡志刚, 赵福运).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(2): 209. [13] REN Yu, XU Yi-zhuang, WANG Jing, ZHANG Yuan-fu, WANG Fan, SHI Jing-sen, WU Jin-guang(任 予, 徐怡庄, 王 晶, 张元福, 王 凡, 石景森, 吴瑾光).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(4): 681.
