Abstract:In the past few years extensive attention has turned toward optical biopsy, because of its ability to perform noninvasive, real time and accurate diagnosis of tissue pathologies, especially precancer.Fluorescence spectroscopy is one of a series of techniques widely developed for optical biopsy, and can be generally divided into two categories: fluorescence spectroscopy and imaging.This paper reviews the applications of autofluorescence and exogenous fluorescence to optical biopsy, and the principle and advantages as well as the clinic applications of various steady-state and time-resolved fluorescence spectroscopy and imaging techniques.Furthermore, the development perspectives of optical biopsy by using fluorescence spectroscopy and imaging are also briefly discussed.
[1] Raloff Janet.Science News, 2001, 159(14): 214. [2] Wagnieres G A,Star W M, Wilson B C.Photochemistry and Photobiology, 1998, 68(5): 603. [3] Rebecca Richards-Kortum,Eva Sevick-Muraca.Annu.Rev.Phys.Chem., 1996, 47: 555. [4] Meyers R A.Encyclopedia of Analytical Chemistry.John Wiley and Sons Ltd, Chichester, 2000. [5] Utzinger Urs,Trujillo E V, Atkinson E N.IEEE Transactions on Biomedical Engineering, 1999, 46(11): 1293. [6] Gregory M Palmer,Crystal L Marshek, Kristin M Vrotsos, et al.Lasers in Surgery and Medicine, 2002, 30: 191. [7] Rebekah Drezerk,Konstantin Sokolov, Utzinger Urs, et al.Journal of Biomedical Optics, 2001, 6(4): 385. [8] Sung K Chang,Michele Follen, Anais Malpica, et al.IEEE Transactions on Biomedical Engineering, 2002, 49(10): 1102. [9] Wesley M Sharman,Cynthia M Allen, Johan E Van Lier.Drug Discovery Today, 1999, 4(11): 507. [10] XU De-yu(许德余).Chin.J.Laser Med.Surg.(中国激光医学杂志), 2001, 10(2): 44. [11] LI Bu-hong, XIE Shu-sen, LU Zu-kang(李步洪,谢树森,陆祖康).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(6): 373. [12] XIE Shu-sen, LI Bu-hong, LI Hui, et al(谢树森,李步洪,李 晖,等).Chinese Journal of Lasers(中国激光), 2001, B10(4): 310. [13] QU Jun-le, NIU Han-ben, GUO Bao-ping(屈军乐, 牛憨笨,郭宝平).Acta Photonica Sinica(光子学报), 1997, 26(9): 809. [14] Cubeddu R,D Comelli, Andrea C D, et al.Journal of Physics D: Applied Physics, 2002, 35: 61. [15] Jean-Michel I Maarek, Laura Marcu, Michael C Fishbein, et al.Lasers in Surgery and Medicine, 2000, 27: 241. [16] Jain B, Majumder S K, Gupta P K.Lasers in the Life Sciences, 1998, 8: 163. [17] LI Bu-hong, XIE Shu-sen, LU Zu-kang(李步洪,谢树森,陆祖康).Optoelectronics·Laser (光电子·激光), 2002, 13(10): 1071. [18] Thomas Glanzmann, Jean-Pierre Ballini, Hubert van den Bergh, et al.Review of Scientific Instruments, 1999, 70(10): 4067. [19] LI Bu-hong, LU Zu-kang, XIE Shu-sen(李步洪,陆祖康,谢树森).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(2): 331. [20] Rinaldo Cubeddu, Pifferi A, Taroni P, et al.IEEE Journal of Selected Topics in Quantum Electronics, 1999, 5(4): 923.