Abstract：An energy transfer technique between acridine orange (AO) and rhodamine 6G (R6G) was studied, and the optimum experimental conditions of energy transfer were defined.It was found that the effective energy transfer could occur between AO and R6G in the dodecylbenzene sodium sulfonate solution with Na2HPO4-citric acid buffer solution at pH 5.0.The fluorescence intensity of AO-R6G system was diminished by vitamin B12 in an alkalescence medium.Based on the AO-R6G energy transfer system a novel fluorescence quenching method for the determination of vitamin B12 has been developed.Under optimal conditions, the linear range of calibration curves for the determination of vitamin B12 was 0-3.0×10-5 mol·L-1.The detection limits were 4.8×10-7 mol·L-1 for Vitamin B12.Among six times of determination, the relative standard deviation was 0.51%-0.64%, and the recovery was 98.40%-103.62%.The method features good recurrence, rapidity of reaction, good stability, and few interfering substances.It can be satisfactorily used in the determination of the injection content of vitamin B12.
刘保生，高 静，杨更亮. 吖啶橙-罗丹明6G能量转移荧光猝灭法测定维生素B12[J]. 光谱学与光谱分析, 2005, 25(07): 1080-1082.
LIU Bao-sheng, GAO Jing, YANG Geng-liang. Determination of Vitamin B12 Concentration by Fluorescence Quenching with Acridine Orange-Rhodamine 6G Energy Transfer System. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(07): 1080-1082.
[1] ZHANG Xin-yan, YANG Ming-hua, ZHENG Yun-fa(张鑫燕, 杨明华, 郑云法).Chemical Reagents(化学试剂), 2002, 24(2): 99. [2] SUO Zhi-rong, ZHANG Hong-fang, YU Hao, et al(索志荣, 张宏芳, 于 浩，等).Chinese Journal of Applied Chemistry(应用化学), 2002, 19(4): 381. [3] LUO Yi, HAO Chang-ming(罗 , 郝常明).China Food Additives(中国食品添加剂), 2002, 3: 15. [4] ZHAO Xiao-jie, JIANG Shan, FAN Yong-chang, et al(赵晓杰, 江 山, 范永昌，等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1994, 14(1): 29. [5] GAO Feng, ZHU Chang-qing, WANG Lun(高 峰，朱昌清，王 伦).Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(1)： 85. [6] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, XU Jin-gou, WANG Zun-ben(陈国珍, 黄贤智, 郑朱梓，许金钩，王尊本).Fluorescence Analytical Methods(Second Edition)(荧光分析法·第2版).Beijing： Science Press (北京： 科学出版社), 1990.122. [7] The Officinal Committee of the Hygiene Department in the People's Republic of China(中华人民共和国卫生部药典委员会编).Pharmacopoeia of the People's Republic of China(中华人民共和国药典).Beijing： Chemical Industry Press(北京： 化学工业出版社), 1985.493. [8] The Medicinal Industrial Institute of Shanghai(上海医药工业研究所编).The Industrial Analysis of Antibiotic(抗生素工业分析).Beijing： Chinese Medicine Scientific and Technological Press(北京： 中国医药科技出版社), 1991.422.
