1. Laboratory of Biological Science, Zhejiang Normal University, Jinhua 321004, China 2. The Administration Bureau of Dapanshan Natural Reserve, Suichang 323300, China
Abstract：Ulmus elongata, an endemic species in China, is one of the grade Ⅱ national key conservation rare and endangered plants. The spectra of root, stem, skin and leaf of Ulmus elongata sampled from eight different sites were determined by Fourier transform infrared (FTIR) spectrometry with OMNI-sampler directly, fast and accurately. A positioning technology of OMNIC E.S.P.5.1 intelligent software and ATR correction was used. The background was scanned before the determination of every example. The peak value and absorbance were ascertained using a method of baseline correction in infrared spectra, and then the relativity between absorption peaks of the spectra and the soil nitrogen was analyzed. Results from the comparison of the spectra showed some differences in their FTIR spectra among root, stem, skin and leaf of Ulmus elongata from the same plant. The coefficients of correlation between chemical composition of this four different organs of Ulmus elongata and soil nitrogen were positive in different degrees. There was the significantly positive correlation between chemical composition of stem and total nitrogen (p<0.05). When the wave-number was 3 365 cm-1, there was a significantly positive correlation between chemical composition of skin and total nitrogen, and a low correlation between root and leave chemical composition and total nitrogen. There was also a certain extent correlation between chemical composition of this four different organs of Ulmus elongata and soil available nitrogen, but the coefficients of correlation was smaller, and the level of the statistic significance was not significant (p>0.05). It was showed that the change in soil total nitrogen has some influence on chemical composition of different organs of Ulmus elongata, but the degree of available nitrogen was very smaller. The linear correlation between soil total nitrogen and organs chemical composition of Ulmus elongate, not only provided the theoretic basis for plant nutriology and nutrient ecology of Ulmus elongate, but also proved that the plants and soil were inseparable. The results also showed that FTIR can be used widely for analysis of the correlation between chemical composition of endangered plants and soil physical and chemical properties in the future, and indicated that the new method has practicability and reliability to a certain degree.
[1] FU Li-guo, CHEN Jia-rui, TANG Yan-cheng(傅立国, 陈家瑞, 汤彦承). Acta Phytotaxonomica Sinica(植物分类学报), 1979, 17(1): 45. [2] ZOU Gao-shun(邹高顺). China Forestry Science and Technology(林业科技开发), 1995, (1): 2. [3] Institute of Botany;Chinese Academy of Sciences(中国科学院植物研究所编). Rare and Endangered Plants in China(中国珍稀濒危植物). Shanghai: Shanghai Education Press(上海: 上海教育出版社), 1989. 351. [4] Armesto J J, Pickett S T, McDonnell M J. Spatial Heterogeneity During Succession: A Cyclic Model of Invasion and Exclusion, Ecological Heterogeneity. New York: Spring Verlag, 1991. 256. [5] LI Jian-zhou, SHA Li-qing, WANG Jun, et al(李检舟, 沙丽清, 王 君, 等). Journal of Mountan Science(山地学报), 2006, 24(2): 186. [6] WAN Kai-yuan, CHEN Fang, LI Zuo-zhou, et al(万开元, 陈 防, 李作洲, 等). Ecology and Environment(生态环境), 2004, 13(2): 261. [7] Griffiths P R, De Haseth J A. Fourier Transform Infrared Spectroscopy. New York: Wiley-Interscience. 1986. [8] LI Yan, WU Ran-ran, YU Bai-hua, et al(李 燕, 吴然然, 于佰华, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(10): 1846. [9] ZHAO Hua-rong, WEN Shu-min, WANG Xiao-yan, et al(赵花荣, 温树敏, 王晓燕, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(5): 705. [10] JIN Xiang-jun, LI Xiao-ping, LIU Zhi-qiang, et al(金向军, 李晓萍, 刘志强, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(4): 614. [11] HONG Qing-hong, CHENG Ze-feng, CHENG Cun-gui(洪庆红, 成则丰, 程存归). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(9): 1610. [12] ZHANG Chang-jiang, LI Dan-ting, LIANG Jiu-zhen, et al(张长江, 李丹婷, 梁久祯, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(1): 50. [13] Kim S W, Ban S H, Cho S, et al. Plant Cell Reports, 2004, 23: 246. [14] GUO Shui-liang, LI Pei-ling, FANG Fang, et al(郭水良, 李佩玲, 方 芳, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(5): 693. [15] HAO Chao-yun, CHENG Cun-gui, LIU Peng(郝朝运, 程存归, 刘 鹏). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(1): 38. [16] WEI Liang-ming, JIANG Hai-ying, LI Jun-hui, et al(魏良明, 姜海鹰, 李军会, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(9): 1404. [17] HONG Qing-hong, LI Dan-ting, HAO Chao-yun(洪庆红, 李丹婷, 郝朝运). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(8): 1246. [18] CHEN Yun-ping, CHEN Rui-qiang, CHENG Xian-su, et al(陈云平, 陈瑞强, 程贤, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(10): 1880. [19] RUI Yu-kui, HUANG Kun-lun, WANG Wei-min, et al(芮玉奎, 黄昆仑, 王为民, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(12): 219.0
