Study on the Membrane Protein Conformational Changes and Mechanisms of Myocardial Cell Irradiated by Pulse Microwave
DENG Hua1, SONG Zhan-jun2, WANG De-wen3, YANG Hong1, MA Chun-quan1, LU Yu-kui1
1. College of Life Science, Foshan Science Technology University, Foshan 528231, China 2. National Center of Biomedical Analysis, Beijing 100850, China 3. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
Abstract：Micro-Fourier transform infrared spectroscopy (FTIR) technique was applied to study the membrane protein conformational and functional changes of myocardial cell irradiated by pulse microwave. The results show that pulse microwave could influence the membrane protein structure markedly. The stretching vibration of lipid —CH2—, lecithoid CO, amide Ⅰ and Ⅱ region was decreased or displaced. The secondary structures of membrane protein were also changed by irradiation. The percentage of α-helix and β-pleated sheet structure decreased remarkably, and the disordering of secondary membrane proteins increased. All the above changes are correlated with the irradiation dosage. The results indicated that the integrality of myocardial cell membrane was injured by pulse microwave, and the membrane fluidity and stability decreased. Multi-biochemically active structures were damaged. Then all the changes could make a biochemical foundation of pathologic effects, which included membrane function decline, cell morphological change, configuration injuring and apoptosis etc. This paper is from a new view of protein conformation to explore the molecular pathologic mechanism of the damage caused by pulse microwave irradiation.
邓桦1,宋占军2 ,王德文3,杨鸿1,马春全1,卢玉葵1 . 脉冲微波辐照影响心肌细胞膜蛋白构象及其机制的研究[J]. 光谱学与光谱分析, 2006, 26(08): 1437-1440.
DENG Hua1, SONG Zhan-jun2, WANG De-wen3, YANG Hong1, MA Chun-quan1, LU Yu-kui1 . Study on the Membrane Protein Conformational Changes and Mechanisms of Myocardial Cell Irradiated by Pulse Microwave . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(08): 1437-1440.
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