FTIR Characterization of the Secondary Structure of a Staphylokinase Variant (K35R) Encapsulated within PLGA Microspheres
HE Jin-tian1,2, WANG Gai-zhen3, SONG Hou-yan1,2
1. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China 2. Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, Shanghai 200032, China 3. College Laboratory Environmental Science and Engineering, Hebei University of Science and Technology Shijiazhuang 050018, China
Abstract:The secondary structure of a staphylokinase variant (K35R, DGR) encapsulated in poly(lactic-co-glycolic acid) (PLGA) microspheres was quantitatively examined by Fourier transform infrared (FTIR) spectroscopy. Resolution enhancement technique and Fourier deconvolution were combined with band with curve-fitting procedures to quantitate the spectral information from the amide Ⅰ bands. Nine component bands were found under the broad, nearly featureless amide Ⅰ bands and assigned to α-helix, β-sheet, turn and irregular(random) structures. The changes of bands at 1 651 and 1 623 cm-1 after encapsulation were discussed.
贺进田1,2,王改珍3, 宋后燕2 . 傅里叶变换红外光谱法研究PLGA微球中葡激酶突变体(K35R)的二级结构[J]. 光谱学与光谱分析, 2006, 26(07): 1209-1212.
HE Jin-tian1,2, WANG Gai-zhen3, SONG Hou-yan1,2. FTIR Characterization of the Secondary Structure of a Staphylokinase Variant (K35R) Encapsulated within PLGA Microspheres. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(07): 1209-1212.
[1] Susi H, Byler D M. Biochem. Biophys. Res. Commun., 1983, 115(1): 391. [2] Byler D M, Susi H. Biopolymers, 1986, 25(3): 469. [3] Susi H, Byler D M. Methods Enzymol., 1986, 130: 290. [4] Yang T H, Dong A, Meyer J, et al. J. Pharm. Sci., 1999, 88(2): 161. [5] Carrasquillo K G, Costantino H R, Cordero R A, et al. J. Pharm. Sci., 1999, 88(2): 166. [6] Fu K, Griebenow K, Hsieh L, et al. J. Control Release, 1999, 58(3): 357. [7] Su H B, Zhang Y G, He J T, et al. Acta Biochim. Biophys. Sin., 2004, 36: 336. [8] Dornberger U, Fandrei D, Backmann J, et al. Biochim. Biophys. Acta, 1996, 1294(2): 168. [9] Fabian H, Naumann D, Misselwitz R, et al. Biochemistry, 1992,31(28): 6532. [10] Andreeva A E, Karamancheva I R, J. Mol. Struct., 2001, 565-566: 177. [11] Dong A, Prestrelski S J, Allison S D, et al. J. Pharm. Sci., 1995, 84: 415. [12] Perez C, De Jesus P, Griebenow K. Int. J. Pharm., 2002, 248(1-2): 193.