FTIR Spectroscopic Study on Gallbladder Carcinoma Cell and Nucleus
SUN Xue-jun1, SUN Feng-lei1,DU Jun-kai1,PAN Qing-hua2,ZHANG Yuan-fu2,XU Yi-zhuang2, SHI Jing-sen1*, WU Jin-guang2
1. The First Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China 2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Abstract：The aim of the present research is to establish the cell basis for the carcinoma tissue diagnosis by exploring a method to obtain the FTIR (Fourier transform infrared spectra) of the cultured carcinoma cell and nucleus with FTIR spectroscopy, and investigating the special spectral features of the carcinoma cell and nucleus compared with the carcinoma tissues. In this paper, the gallbladder carcinoma tissues confirmed by histology were measured using a Nicolet Magna 5700-Ⅱ FTIR spectrometer and the corresponding FTIR spectra were obtained. The cultured gallbladder carcinoma cell (GBC-SD) and nucleus were centrifuged to provide a small pellet of cell and nucleus for FTIR analysis. The cell and nucleus pellet was then placed on the OMNIC sampler. Then the infrared spectra were recorded by the same equipment. Based on the previously established criteria, a comparative study was subsequently carried out between the spectra of the cultured carcinoma cell and nucleus (GBC-SD) and that of the corresponding gallbladder tissues. Several infrared spectral features of the carcinoma cell and nucleus were obtained. All the results suggest that the spectral features of the carcinoma cell and nucleus can be well reflected by that of the carcinoma tissue, though the later is more complicated, which might originate from the intrinsic complexity of the tissue. This study shows that the diagnosis of carcinoma tissue by FTIR method exhibits sufficient cell basis.
孙学军1,孙丰雷1,杜俊凯1,潘庆华2,张元福2,徐怡庄2,石景森1*,吴瑾光2. 傅里叶变换红外光谱用于胆囊癌细胞及其细胞核结构的基础研究[J]. 光谱学与光谱分析, 2009, 29(07): 1750-1753.
SUN Xue-jun1, SUN Feng-lei1,DU Jun-kai1,PAN Qing-hua2,ZHANG Yuan-fu2,XU Yi-zhuang2, SHI Jing-sen1*, WU Jin-guang2. FTIR Spectroscopic Study on Gallbladder Carcinoma Cell and Nucleus. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(07): 1750-1753.
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