Chip-Based Capillary Electrophoresis On-Line Coupled with Atomic Fluorescence Spectrometry and Its Application to Rapid Speciation Analysis
LI Feng1, WANG Dong-dong1, YAN Xiu-ping1*, SU Rong-guo2, LIN Jin-ming2
1. Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China 2. Research Center for Eco-Environment, Chinese Academy of Sciences, Beijing 100085, China
Abstract：Chip-based capillary electrophoresis (chip-CE) is under rapid progress due to its high efficiency, fast separation, little sample consumption and integration. Due to the short optical path (30-50 μm), the conventional UV absorbance cannot satisfy the chip detection needs. Atomic fluorescence spectrometer(AFS) is a selective and sensitive detector for hydride-forming elements. With the strong points of low cost and easy operation, AFS is attractive as an ideal detector of chip-CE for mercury, arsenic and selenium, etc. A few important practical aspects of coupling chip-CE to AFS are critically discussed. These include the design of the chip, chip-AFS interface, gas-liquid separator, AFS atomizer, and elimination of the effect of backpressure on separation. The application of the developed chip-CE-AFS to the mercury speciation analysis is highlighted.
李峰1,王冬冬1,严秀平1*,苏荣国2,林金明2 . 芯片毛细管电泳-原子荧光在线联用设计[J]. 光谱学与光谱分析, 2006, 26(06): 1158-1161.
LI Feng1, WANG Dong-dong1, YAN Xiu-ping1*, SU Rong-guo2, LIN Jin-ming2 . Chip-Based Capillary Electrophoresis On-Line Coupled with Atomic Fluorescence Spectrometry and Its Application to Rapid Speciation Analysis. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(06): 1158-1161.
[1] Vilkner T, Janasek D, Manz A. Anal. Chem., 2004, 76(12): 3373. [2] WANG Shi-li, FANG Zhao-lun(王世立, 方肇伦). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2000, 20(2): 143. [3] Prest J E, Baldock S J, Fielden P R, et al. Anal. Bioanal. Chem., 2003, 376(1): 78. [4] Prest J E, Baldock S J, Fielden P R, et al. J. Chromatogr. A, 2003, 990(1-2): 325. [5] Song Q J, Greenway G M, McCreedy T. J. Anal. At. Spectrom., 2003, 18(1): 1. [6] Song Q J, Greenway G M, McCreedy T. J. Anal. At. Spectrom., 2004 , 19(7): 883. [7] Li F, Wang D D, Yan X P, et al. Electrophoresis, 2005, 26(11): 2261. [8] YIN Xue-bo, JIANG Yan, YAN Xiu-ping, et al(尹学博, 江 焱, 严秀平, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2004, 25(4): 618. [9] Fan Z H, Harrison D J. Anal. Chem., 1994, 66(1): 177. [10] Yin X B, Yan X P, Jiang Y, et al. Anal. Chem., 2002, 74(15): 3720.
