Abstract：A fast fluorescence micro-imaging system using mainly intensified charge couple device(ICCD), argon-ion laser, and xenon lamp was set up, and its preliminary application in living cells was presented. Real-time observation and imaging of fast concentration and distribution changing of intracellular Ca2+ labeled by Fluo-3, a fluorescent Ca2+ indicator, in the proliferation process of rat cerebral micro-vessels endothelial cells(rCMECs) were carried out, and curves of artificial intensity versus imaging sequence of four typical points were obtained. It is shown that the ICCD-based fast fluorescence micro-imaging system is a powerful tool for recording the real-time fast processes in living cells.
林丹樱，孙云旭，马万云*，韩 东 . 基于ICCD的快速荧光显微成像技术及在活细胞研究中的初步应用[J]. 光谱学与光谱分析, 2006, 26(05): 917-921.
LIN Dan-ying, SUN Yun-xu, MA Wan-yun*, HAN Dong. ICCD-Based Fast Fluorescence Micro-Imaging Technique and Preliminary Application in Living Cells . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(05): 917-921.
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