Spectroscopic Investigation of the Influence of Calcium Ion on the Structures of Casein Micelles
WANG Peng-jie1, WU Jian-ping2, ZHANG Hao1, GUO Hui-yuan1, LIU Hong-na3, REN Fa-zheng1*
1. Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China 2. University of Alberta, Edmonton, Canada 3. College of Food Science and Technology, Gansu Agricultural University, Lanzhou 630070, China
Abstract：The effects of calcium ion on the structural properties of casein micelles in the course of heat treatment were synthetically examined by non-structure-invasive spectrometry. The hydrophobicity, reflected by extrinsic fluorescence (ANS fluorescence), was positively correlated with the concentration of the calcium ion, within the range of 0 to 12 mmol·L-1. Meanwhile, the turbidity and stability of casein micelles also increased with the growth of calcium concentrations. However, opposite results were observed for hydrodynamic diameter and polydispersity index. Compared with the calcium ion, the calcium-chelator (citrate) has an opposite effect on the structural characteristics of casein micelles. Within the calcium concentrations range of 0 to 12 mmol·L-1, the hydrophobicity, stability and turbidity were negatively correlated with the concentration of the calcium ion, nevertheless, opposite results were observed for hydrodynamic diameter and polydispersity index. All the results indicate that the calcium ion could be used to modify the structures of casein micelles during heat heatment.
王鹏杰1，吴建平2，张 昊1，郭慧媛1，刘洪娜3，任发政1* . 钙离子对酪蛋白胶束结构影响的光谱学研究 [J]. 光谱学与光谱分析, 2014, 34(01): 92-97.
WANG Peng-jie1, WU Jian-ping2, ZHANG Hao1, GUO Hui-yuan1, LIU Hong-na3, REN Fa-zheng1* . Spectroscopic Investigation of the Influence of Calcium Ion on the Structures of Casein Micelles . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(01): 92-97.
[1] Salaon F, Mietton B, Gaucheron F. Milchwissenschaft, 2007, 62(1): 20. [2] Singh H, Roberts M S, Munro P A, et al. Journal of Dairy Science, 1996, 79(8): 1340. [3] Wang P, Liu H, Wen P, et al. International Dairy Journal, 2013, 31(2): 107. [4] Fox P F, Brodkorb A. International Dairy Journal, 2008, 18(7): 677. [5] Yüksel Z, Erdem Y K. Journal of Food Engineering, 2005, 67(3): 301. [6] Gebhardt R, Doster W, Friedrich J, et al. European Biophysics Journal, 2006, 35(6): 503. [7] Chakraborty A, Basak S. Journal of Photochemistry and Photobiology B: Biology, 2007, 87(3): 191. [8] Anema S G. Food Chemistry, 2009, 114(1): 161. [9] Lencki R W. Journal of Dairy Science, 2007, 90(1): 75. [10] Anema S G, Li Y. Lebensmittel-Wissenschaft und-Technologie, 2000, 33(5): 335. [11] Partschefeld C, Schwarzenbolz U, Richter S, et al. Biotechnol. J., 2007, 2(4): 456. [12] Tran Le T, Saveyn P, Hoa H D, et al. International Dairy Journal, 2008, 18(12): 1090. [13] Beliciu C M, Moraru C I. Journal of Dairy Science, 2009, 92(5): 1829.
