1. Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100094, China 2. Department of Environmental Science and Engineering, College of Tourism, Shanghai Normal University, Shanghai 200234, China
Abstract：The Resonance light scattering (RLS) spectra, fluorescence spectra, and absorption spectra of Cal-Red-Cu(Ⅱ) metal complex with bovine serum albumin(BSA) were studied. As the Cal-Red-Cu(Ⅱ)-BSA complex formed, the RLS spectra and fluorescence spectra enhanced, but the absorption spectra weakened and the absorption peak at 594 nm disappeared. At pH 5.65-5.75，the enhanced RLS of Cal-Red-Cu(Ⅱ) metal complex with BSA system was characterized by the maximum scattering at 317 nm, and the RLS intensity is proportional to the concentration of BSA. Under optimal conditions, the linear range of calibration was 0.75-10 μg·mL-1,the linear regression equation was I=150.88+201.48c(BSA，μg·mL-1)with r=0.997 3, and the limit of detection was 5.62×10-2 μg·mL-1. The method was applied to the determination of BSA in synthetic samples satisfactorily. A mechanism of electrostatic force was suggested for the interaction between BSA and Cal-Red-Cu(Ⅱ).
陈丹丹1,张 涛1,任丽萍1*,石登荣2,于金莲2 . 光谱法研究钙红-Cu(Ⅱ)络合物与蛋白质的相互作用及其应用[J]. 光谱学与光谱分析, 2006, 26(04): 686-689.
CHEN Dan-dan1,ZHANG Tao1,REN Li-ping1*,SHI Deng-rong2,YU Jin-lian2 . Study on the Spectra of Cal-Red-Cu(Ⅱ) Complex with Bovine Serum Albumin and Its Analytical Application. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(04): 686-689.
[1] Liu S F, Yang J H, Wu X, et al. Luminescence, 2004, 19(6): 352. [2] ZHANG Qun, SHEN Yu-hua, XIE An-jian, XIA Bing(张 群, 沈玉华, 谢安建，夏 兵). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002, 22(2): 267. [3] Hu Y J, Liu Y, Shen X S, et al. Journal of Molecular Structure, 2005, 738: 143. [4] ZHONG Hui, LI Na, ZHAO Fenglin, et al. Talanta, 2004, 62: 37. [5] CHEN Yue-hua, LIU Zhong-fang, HU Xiao-li, LIU Shao-pu(陈粤华，刘忠芳，胡小莉，刘绍璞). Chinese Journal of Analytical Chemistry(分析化学)，2005, 33: 802. [6] JIA Run-ping, DONG Li-jun, LI Qian-feng, et al. Analytica Chimica Acta, 2001, 442: 249. [7] Dong L J, He J, Li Q F, et al. Analytical Biochemistry, 2003, 315: 22. [8] YAN Cheng-nong, ZHANG Hua-xin, JU Xiang, et al(颜承农，张华新，鞠 香，等). Chinese Journal of Analytical Chemistry(分析化学)，2005, 33(6): 759. [9] WEI Yong-ju, LI Ke-an, TONG Shen-yang(魏永巨，李克安，童沈阳). Chemical Journal of Chinese Universities(高等学校化学学报)，1994, 15(10): 1470. [10] HU Qiu-luan, LI Ke-an(胡秋娈，李克安). Acta Scientiarum Naturalium Universitatis Pekinensis(北京大学学报·自然科学版), 1999, 35(6): 850.
