Research on the Interaction of Aristolochic Acid and Bovine Serum Albumin
LIU Jia-qin1, 2,TIAN Jian-niao3,BIAN Qing-quan1,HU Zhi-de2
1. Department of Chemistry, Mianyang Teachers College, Mianyang 621000, China 2. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China 3. College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541001, China
Abstract：The interaction between aristolochic acid and bovine serum albumin(BSA) under physiological conditions was investigated by fluorescence quenching methods. The results indicate that there is a strong interaction between aristolochic acid and BSA, and the distances between the binding location and tryptophan residue is 2.8 nm. The binding location of aristolochic acid in BSA is in subdomain ⅢA. In addition, the effects of aristolochic acid on the protein second structure were studied using CD and FTIR techniques. The results of CD proved that the α-helix contents of BSA decreased from 43.5% to 36.7% after binding with aristolochic acid.
刘家琴1, 2,田建袅3,边清泉1,胡之德2 . 马兜铃酸与牛血清白蛋白的相互作用研究[J]. 光谱学与光谱分析, 2006, 26(04): 715-719.
LIU Jia-qin1, 2,TIAN Jian-niao3,BIAN Qing-quan1,HU Zhi-de2 . Research on the Interaction of Aristolochic Acid and Bovine Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(04): 715-719.
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