Determination of Methylmercury in Biological and Sediment Samples by Capillary Gas Chromatography On-Line Coupled with Atomic Fluorescence Spectrometry
SHI Jian-bo,LIAO Chun-yang,WANG Ya-wei,JIANG Gui-bin*
Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
Abstract：A comprehensive method for the determination of methylmercury by capillary gas chromatography (GC) on-line coupled with atomic fluorescence spectrometry (AFS) was developed. The hyphenation system was carefully designed and the operation conditions such as injector temperature, carrier gas flow rate, make-up gas flow rate and argon flow rate were optimized in detail. The absolute detection limits (3σ) were 0.005 ng of Hg for both methylmercury (MMC) and ethylmercury (EMC). The relative standard deviations (RSD, n=5) for MMC and EMC at 10 ng·mL-1 of Hg levels were 2.5% and 1.3%, respectively. The method was evaluated by analyzing a certified reference material(DORM-2), and the MMC concentration determined was in good agreement with the certified value. In addition, the recoveries of MMC in two sediment samples were 70% and 77%, respectively. The proposed method was accurate, sensitive and suitable for the determination of MMC in biological and sediment samples.
Key words：Capillary gas chromatography;Atomic fluorescence spectrometry;Methylmercury;Biological samples;Sediments
史建波, 廖春阳, 王亚伟, 江桂斌*. 气相色谱和原子荧光联用测定生物和沉积物样品中甲基汞[J]. 光谱学与光谱分析, 2006, 26(02): 336-339.
SHI Jian-bo,LIAO Chun-yang,WANG Ya-wei,JIANG Gui-bin*. Determination of Methylmercury in Biological and Sediment Samples by Capillary Gas Chromatography On-Line Coupled with Atomic Fluorescence Spectrometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(02): 336-339.
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