Feasibility of Using NIR Spectroscopy to Detect Melamine in Milk
DONG Yi-wei1, TU Zhen-hua2, ZHU Da-zhou2, LIU Ya-wei1, WANG Ya-nan1, HUANG Jin-li1, SUN Bao-li1, FAN Zhong-nan1*
1. Institute of Environment and Sustainable Development in Agriculture, The Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Environment & Climate Change, Ministry of Agriculture, Beijing 100081, China 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Abstract：In the present study, 22 certified milk samples without melamine were collected, then 50 adulterated milk samples with added different content of melamine (0.1-1 500 mg·kg-1) were prepared. The near-infrared (NIR) spectra of these milk samples were measured. The possibility of using NIR spectra to detect melamine in milk was studied. Partial least square regression (PLSR) was applied to construct the calibration model between NIR spectra and the content of melamine. The results showed that NIR spectroscopy can not accurately predict the content of melamine because of its poor detection limit. However, the combination of NIR spectra and partial least square-discriminate analysis (PLS-DA) was applied to differentiate the certified milk samples and the adulterated milk sample. The classification accuracy was 100%. Therefore, NIR spectra could be used to preliminarily detect whether the milk was adulterated with melamine. As a complementary detecting method to the high performance liquid chromatography (HPLC), NIR spectra could improve the detecting efficiency of milk.
董一威1，屠振华2，朱大洲2，刘亚伟1，王亚男1，黄金丽1，孙宝利1，范中南1* . 利用近红外光谱快速检测牛奶中三聚氰胺的可行性研究[J]. 光谱学与光谱分析, 2009, 29(11): 2934-2938.
DONG Yi-wei1, TU Zhen-hua2, ZHU Da-zhou2, LIU Ya-wei1, WANG Ya-nan1, HUANG Jin-li1, SUN Bao-li1, FAN Zhong-nan1*. Feasibility of Using NIR Spectroscopy to Detect Melamine in Milk. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(11): 2934-2938.
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