Simultaneous Determination of Ten Active Components in Qingkailing Injection and Relationship with Quality Control
WU Yan-shu1,2, JIN Cheng1, ZHANG Qian1,2, JIAO Yi3, YAN Dan1, XIAO Xiao-he1*, DU Xiao-xi4, WANG Jian2
1. PLA Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, China 2. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China 3. Pharmacy College, Hunan University of Traditional Chinese Medicine, Changsha 410002, China 4. Centre for Drug Evaluation, State Food and Drug Administration, Beijing 100038, China
Abstract：A method was established for simultaneous determination of ten active components in Qingkailing injection (QKL), and could be used as a feasible method for the quality control of QKL. HPLC-DAD-ELSD was employed for the determination at the optimal wavelength (255 nm). Fifteen samples of QKL at different batches from different factories were analyzed by the means. The results show that the method is corresponding with specified standard. The contents from different samples (QKL from different factories and different batches) have great disparity (The RSD values range from 2.17% to 65.54%). It is concluded that the method is reliable and convenient for the quality control of QKL. The key point of QKL quality control is to control the chief activity components as much as possible.
Key words：Qingkailing injection;HPLC-DAD-ELSD;Simultaneous determination;Ten active components;Quality control
武彦舒1,2，金 城1，张 倩1,2，焦 懿3，鄢 丹1，肖小河1*，杜晓曦4，王 建2 . 清开灵注射液中10种有效成分的同时测定及其质量相关性研究[J]. 光谱学与光谱分析, 2009, 29(11): 3112-3116.
WU Yan-shu1,2, JIN Cheng1, ZHANG Qian1,2, JIAO Yi3, YAN Dan1, XIAO Xiao-he1*, DU Xiao-xi4, WANG Jian2 . Simultaneous Determination of Ten Active Components in Qingkailing Injection and Relationship with Quality Control . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(11): 3112-3116.
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