Application of FTIR and Active Ingredients Quantitative Analysis on Quality Control of Dai Medicine Alstonia scholaris (L.) R. Br.
YANG Ni-na1, 2, YANG Chun-yong3, WANG Yuan-zhong4,5*, ZHAO Ying-hong1*
1. Dai Hospital of Xishuangbanna Dai Autonomous Prefecture, Xishuangbanna 666100, China 2. College of TCM, Yunnan University of TCM, Kunming 650500, China 3. Yunnan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Xishuangbanna 666100, China 4. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China 5. Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China
Abstract：FTIR fingerprint of the leaves and immature stems of Alstonia scholaris (L.) R. Br. was established as a content determination method for the detection of picrinine, ursolic acid and oleanolic acid. Different medicinal parts were identified based on principal component analysis, while exploring the influence of immature stems for the leaves and the application of FTIR and HPLC in the Dai quality control in order to speed up the pace of Dai medicine modernization. Infrared spectroscopy of different batches samples were collected and the data was preprocessed as to automatic baseline correction, smooth, ordinate normalization, second order derivative, and then to PCA, all the datum in triplicate. For content determination of picrinine, mobile phase was acetonitrile (40) water (contain 0.1% ammonia water) (60) and the wavelength was set at 287 nm. For ursolic acid and oleanolic acid, the mobile phase was mixture (12∶88) of 0.1% formic acid in water (A) and methanol (B). Wavelength was 210 nm. As the results, the original spectrum difference was not obvious for leaves and stems. Pretreatment spectroscopy had a significant variation on absorption peak number and intensity in 3 000～2 800 and 1 800～500 cm-1. The results of PCA showed that, the leaves and stems were separated; in addition the difference of different batches leaves was bigger than the stems. The mean contents of picrinine, ursolic acid and oleanolic acid in leaves were 0.79，8.47，7.51 and 0.21，1.78，1.67 mg·g-1 in stems, respectively. The content of ursolic acid and oleanolic acid is higher than picrinine, but ursolic acid and oleanolic acid content had no obvious difference. Mean content of three ingredients in leaves is much higher than in stems. Picrinine content in leaves was 3.8 times of immature stems, ursolic acid and oleanolic acid content were 5.1 and 4.2 times of immature stems, respectively. The variety of picrinine content in different batches samples was biggest, ursolic acid and oleanolic acid content was relatively stable. The overall quality of leaves has an obvious difference compared with the immature stems, so the leaves of A. scholaris mix with immature stems could not be as Dai medicine in Dai clinic. Infrared spectroscopy combined with chromatography can quickly identify different medicinal parts and evaluate overall quality of Dai medicine, which can apply to quality control of Dai medicine.
Key words：FTIR fingerprint;Dai medicine;Alstonia scholaris (L.) R. Br.;Picrinine;Ursolic acid;Oleanolic acid;Quality control
杨妮娜1, 2，杨春勇3，王元忠4, 5*，赵应红1* . FTIR结合有效成分定量分析在傣药灯台叶质量控制中的应用 [J]. 光谱学与光谱分析, 2017, 37(01): 58-64.
YANG Ni-na1, 2, YANG Chun-yong3, WANG Yuan-zhong4,5*, ZHAO Ying-hong1* . Application of FTIR and Active Ingredients Quantitative Analysis on Quality Control of Dai Medicine Alstonia scholaris (L.) R. Br. . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(01): 58-64.
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