Abstract：Fourier transform infrared spectroscopy (FTIR) provides biochemical profiles containing overlapping signals from a majority of the compounds that are present when whole samples are analyzed. The spectra of the xylem of 112 samples belonging to 12 species and 5 genera in Caprifoliaceae were determined directly with FTIR spectrometry and OMNI-sampler. Based on the indices of wave number -absorbance, the phylogenetic relationships of the 12 species were analyzed by the methods of principal coordinate analysis (PCO) and cluster analysis. The results showed that the infrared spectra of the 12 plant species are fingerprint-like patterns which are highly typical for different taxa. Genus Heptacodium has close relation with the tribe Linnaeeae, and Lonicereae, showing that its systematic position is probably located between the two tribes. There is no obvious difference between Viburnum and Sambucus, so the authors thought it would be better to put the two genera in Caprifoliaceae. Therefore, the infrared spectra are of taxonomic value at the level of species and genera, and this technique could be widely used for identification and classification of other taxa when standard spectra are available.
郝朝运, 程存归, 刘鹏*. FTIR直接测定法在解决忍冬科一些分类学问题中的应用研究[J]. 光谱学与光谱分析, 2007, 27(01): 38-42.
HAO Chao-yun, CHENG Cun-gui, LIU Peng*. The Applications of Fourier Transform Infrared Spectroscopy in Resolving Some Taxonomic Doubts of Caprifoliaceae. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(01): 38-42.
[1] Muller J. Botany Review, 1981, 47(4): 1. [2] Griffiths P R, de Haseth J A. Fourier Transform Infrared Spectroscopy. New York: Wiley-Interscience. 1986. [3] CHENG Cun-gui, RUAN Yong-ming, LI Bing-lan(程存归, 阮永明, 李冰岚). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(11): 1355. [4] CHENG Cun-gui, SUN Cui-rong, PAN Yuan-jiang(程存归, 孙翠荣, 潘远江). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(9): 1055. [5] CHENG Cun-gui，SHI Hong-qi，ZHU Xiao-jun，et al(程存归, 施红旗, 朱晓军, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(11): 1342. [6] HONG Qing-hong，LI Dan-ting，HAO Chao-yun(洪庆红, 李丹婷, 郝朝运). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(8): 1246. [7] WANG Si-hong, YIN Qi-fan, FAN Yan-ling, et al(王思宏, 尹起范, 范艳玲, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(8): 957. [8] Kim S W, Ban S H, Cho S, et al. Plant Cell Rep, 2004, 23: 246. [9] GUO Shui-liang, LI Pei-ling, FANG Fang, et al(郭水良, 李佩玲, 方 芳, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(5): 693. [10] Metcalfe R. Kew Bulletin, 1952, 247. [11] Donoghue M J. Ecology, 1983, 79: 969. [12] XU Bing-sheng(徐炳声). Acta Phytotaxonomica Sinica(植物分类学报), 1983, 21(1): 26. [13] Takhtajan A L. Systema Magnoliophytorum. Leningrad: Nauka, 1987. 79. [14] TANG Yan-cheng, LI Liang-qian(汤彦承, 李良千). Acta Phytotaxonomica Sinica(植物分类学报), 1994, 32(3): 197. [15] Zhang W H, Chen Z D, Li J, et al. Molecular Phylogenetics and Evolution, 2003, 26: 176. [16] Dahlgren R M T. Botanical Journal of the Linnean Society, 1980, 80: 91. [17] Wilkinson A M. American Journal of Botany, 1949, 36: 481. [18] Section of Palynology of Botany Institute of Chinese Academy of Sciences(中国科学院植物研究所形态室孢粉组). Pollen Morphology of Chinese Plants(中国植物花粉形态). Beijing: Science Press(北京: 科学出版社), 1960. [19] Bohm B A, Glennie C W. Canadian Journal of Botany, 1971, 49: 1799. [20] ZENG Ling-jie, XING Jun-bo, LI Ping(增令杰, 郉俊波, 李 萍). China Journal of Chinese Materia Medicine(中国中药杂志), 2000, 25(3): 184.
