HG-AFS Determination of Selenium in Moringa Oleifera
HUANG Guo-qing1, 2,XIAO Zi-jun2
1. College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China 2. Food Engineering Department, Shaoguan College, Shaoguan 512005, China
Abstract：The Se content in Moringa oleifera was studied by hydride generation atom fluorescence spectrometry(HG-AFS) with wet digestion. The effects of the way of digestion, the work condition of apparatus, the reaction medium and acidity, and the reducing agent and masking agent on the determination of Se were investigated. And the operating condition of apparatus was optimized. The results showed that the detection limit of Se in this method was 0.42 ng·mL-1 in the linear ranger of 0-120 ng·mL-1,the relative standard deviation was 3.53%(n=11), and the recovery of the method was 95.2%-104.6%. It was showed that the method was very sensitive, simple, rapid and accurate.
[1] KONG Xiang-rui(孔祥瑞). Essential Trace Elements (必需微量元素). Hefei: Anhui Scientific and Technical Press(合肥：安徽科学技术出版社)，1982. [2] DUAN Min, WANG Bo(段 敏,王 波). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(8)：1358. [3] National Bureau of Standards of the People’s Republic of China(中华人民共和国国家标准局). Determination of Selenium in Foods, GB/T12399-1996(食品中硒的测定, GB/T12399-1996). Beijing: Chinese Standard Press(北京: 中国标准出版社), 2001. [4] Lü Shui-yuan, DAI Jin-lan, ZHONG Mao-sheng(吕水源，戴金兰，钟茂生). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(7)：1352.
