Study on the Energy Transfer Fluorescence Quenching Reaction of Acridine Orange-4-(2-Pyridylazo) Resorcinol-V(Ⅴ)System and Analytical Application
HOU Ming1,FENG Li-e1,JIANG Zhi-liang1, 2
1. Material and Chemistry Department, Guilin University of Technology, Guilin 541004, China 2. Department of Resource and Environmental Science, Guangxi Normal University, Guilin 541004, China
Abstract：An energy transfer technique for 4-(2-pyridylazo) resorcinol (PAR)-vanadium(Ⅴ)and acridine orange(AO)was studied, and the optimum conditions of energy transfer system were also experimented. It was found that in citrate-Na2HPO4 buffer solution at pH=5.5, energy transfers from AO to vanadium(Ⅴ)-PAR complexes. A new method based on energy transfer fluorescence quenching for the determination of trace vanadium(Ⅴ)with AO-PAR-V(Ⅴ)was established. The equation of linear regression is ΔF=165.4c+2.5, and the determination range of vanadium is 0.012-0.5 μg·mL-1,with detection limit of 0.004 5 μg·mL-1. The correlation coefficient is R=0.998 5, and relative standard deviation is 0.6%. The fluorescence reaction is completed within 15 minutes, and relative fluorescence intensity remains unchanged for 2.5 hours. The influence of foreign ions on the determination of V (0.5 μg· mL-1) was examined, with a related error of 5%. The method has been used in the determination of trace vanadium in biological samples with the relative error of 6.98%, which meets the requirements of trace analysis.
侯明1,冯丽娥1,蒋治良1,2. 吖啶橙-PAR-钒(Ⅴ)能量转移荧光猝灭法的研究及分析应用[J]. 光谱学与光谱分析, 2009, 29(02): 476-478.
HOU Ming1,FENG Li-e1,JIANG Zhi-liang1, 2. Study on the Energy Transfer Fluorescence Quenching Reaction of Acridine Orange-4-(2-Pyridylazo) Resorcinol-V(Ⅴ)System and Analytical Application . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(02): 476-478.
[1] WANG Ping-li, ZHANG Cheng-jiang(王平利，张成江). Computing Techniques for Geophysical and Geochemical Exploration(物探化探计算技术)，2004，26(3)：247. [2] CHEN Lan-hua, GE Hai-yan(陈兰化，葛海燕). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003，23(6)：1154. [3] BAO Suo-yan, LI Shu-cun, SHI Sheng-xun(鲍所言，李书存，石生勋). Chinese Journal of Analytical Chemistry(分析化学)，2001，29(10)：1170. [4] BAO Suo-yan, LI Shu-cun, MENG Fan-hui, et al(鲍所言, 李书存, 孟凡辉, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002，22(2)：284. [5] LIN Shao-yu, GAO Wen-hua, LIU Xiao-xuan, et al(林韶玉，高文华，刘小暄，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2007，27(1)：113. [6] WANG Jin-zhong, LIU Bo, ZAHNG Dong-di, et al(王金中，刘 波，张东娣，等). Chemical Research(化学研究)，2002，13(4)：21. [7] NI Yong-nian, CAI Ying-jun(倪永年，蔡英俊). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(10)：1641. [8] HOU Ming, ZHAO Qing-hua(侯 明 , 赵青华). Chinese Journal of Analysis Laboratory(分析试验室)，2001，20(3)：56. [9] BAO Suo-yan, WANG Gui-hua, LIU Bao-sheng, et al(鲍所言，王桂华，刘保生，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2001，21(1)：87. [10] HOU Ming, ZHANG Jing(侯 明，张 静). Journal of Analytical Science(分析科学学报)，2004，20(6)：619. [11] LIU Bao-sheng, WANG Gui-hua, SUN Han-wen, et al(刘保生，王桂华，孙汉文，等). Chinese Journal of Analytieal Chemistry(分析化学)，2001，29(1)：42. [12] LIU Bao-sheng, GAO Jing, LIU Zhi-chao, et al(刘保生，高 静，刘智超，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(2)：306. [13] LIU Bao-sheng, GAO Jing, YANG Geng-liang(刘保生，高 静，杨更亮). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(7)：1080. [14] LIU Bao-sheng, GAO Jing, YANG Geng-liang(刘保生，高 静，杨更亮). Chinese Journal of Analytieal Chemistry(分析化学)，2005，33(4)：546. [15] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al(陈国珍，黄贤智，郑朱梓，等). Fluorimetry(荧光分析法). Beijing: Science Press(北京：科学出版社)，1990. 112.
