Changes in Organic Carbon Components and Structure of Black Rhizosphere Soil Under Long-Term Different Fertilization
CHEN Lei1, 2, HAO Xiao-yu1, MA Xing-zhu1, ZHOU Bao-ku1, WEI Dan3, ZHOU Lei4, LIU Rong-le5, WANG Hong2*
1. Institute of Soil and Fertilizer and Environment Resources, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
2. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Improving Quality of Arable Land/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Beijing 100081, China
3. Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forest, Beijing 100097, China
4. Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin 150001, China
5. Agro-Environmental Protection Institute of Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Abstract：Soil organic carbon is the crucial driver and regulator of the agricultural ecosystem. In particular, the quantification of rhizosphere organic carbon plays an important role in the soil carbon cycle and mineral nutrient release. Through the research on the changes of organic carbon, labile organic carbon and organic carbon structure in soybean rhizosphere soil under different long-term fertilization, we can further understand the mechanism of rhizosphere organic carbon fixation and stability. The outcome of this research would provide a scientific basis and theoretical support for improving carbon fixation of farmland ecosystem and sustainable development of farmland. The experiment uses a long-term black soil positioning test. Chemical analysis and 13C-NMR were used to study dynamic changes in organic carbon, labile organic carbon and organic carbon structure in soybean rhizosphere soil. The results showed that organic carbon concentrations were generally higher in rhizosphere soil than in bulk soil. Long-term fertilization treatment could significantly increase concentrations of organic carbon and low labile organic carbon in rhizosphere soil, and MNPK treatment had the best effect. Compared with CK treatment, MNPK treatment significantly increased the proportion of alkyl C, O-alkyl C and the ratios of alkyl C to O-alkyl C, and decreased aromatic C and the ratios of aromatic C to total C in rhizosphere soil, especially in bulk soil.NPK treatment increased the proportion of aromatic C and the ratios of aromatic C to total C, increased alkyl C and the ratios of alkyl C to O-alkyl C in rhizosphere soil, and decreased O-alkyl C, which the results were opposite in bulk soil. The above analysis showed that: MNPK treatment significantly increased the content of rhizosphere organic carbon, increased alkyl C, O-alkyl C and the ratios of alkyl C to O-alkyl C while promoting the formation of aggregates and increased the stability of soil particle structure. NPK treatment increased aromatic C, and the ratios of aromatic C to total C reduced the rhizosphere O-alkyl C, which decreased the stability of aggregates. At the same time, it is proved that 13C-NMR technology combined with semi-quantitative analysis could be used to comprehensively analyze the structural changes of different organic carbon functional groups to gain a deeper understanding of the stability mechanism of rhizosphere soil organic carbon.
Key words：Soil organic carbon; Soybean rhizosphere; Black soil; 13C-NMR
陈 磊，郝小雨，马星竹，周宝库，魏 丹，周 磊，刘荣乐，汪 洪. 长期不同施肥处理对黑土根际土壤有机碳结构组分的影响[J]. 光谱学与光谱分析, 2022, 42(12): 3883-3888.
CHEN Lei, HAO Xiao-yu, MA Xing-zhu, ZHOU Bao-ku, WEI Dan, ZHOU Lei, LIU Rong-le, WANG Hong. Changes in Organic Carbon Components and Structure of Black Rhizosphere Soil Under Long-Term Different Fertilization. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3883-3888.
[1] Prather M J, Hohnes C D, Hsu J. Geophysical Research Letters, 2012, 39: 1.
[2] Zhang G B, Ji Y, Ma J, et al. Soil Biology and Biochemistry, 2012, 52: 108.
[3] Maillard , Angers D A. Global Change Biology, 2014, 20: 666.
[4] Kang R F, Ren Y, Wu H J, et al（康日峰, 任 意, 吴会军, 等）. Scientia Agricultura Sinica（中国农业科学）, 2016, 49(11): 2113.
[5] Wang J K, Wang T Y, Zhang X D, et al（汪景宽, 王铁宇, 张旭东, 等）. Journal of Shenyang Agricultural University（沈阳农业大学学报）, 2002, 33(1): 43.
[6] Jones D, Nguyen C, Finlay R D. Plant and Soil, 2009, 321: 5.
[7] Kuzyakov Y. Journal of Plant Nutrition and Soil Science, 2002, 165(4): 382.
[8] Huo C, Luo Y, Cheng W. Soil Biology and Biochemistry, 2017, 111: 78.
[9] Luce M S, Ziadi N, Zebarth B J, et al. Geoderma, 2014, 232-234: 449.
[10] Xu Y H, Chen Z M, Fontaine S, et al. Soil Biology and Biochemistry, 2017, (115): 221.
[11] Assunção S A, Pereira M G, Rosset J S, et al. Science of the Total Environment, 2019, (658): 901.
[12] Dias T, Oakley S, Alarcon-Gutierrez E, et al. Soil Biology and Biochemistry，2013, 58: 163.
[13] Shrestha B, Singh B, Forte C, et al. Soil Use and Management, 2015, 31: 358.
[14] Zhang J J, Cao Z Y, Feng G Z, et al. Pedosphere, 2017, 27(5): 957.
[15] Kundu S, Bhattacharyya R, Prakash V, et al. Soil Tillage Research, 2007, 92: 87.
[16] He Y T, He X H, Xu M G, et al. Soil and Tillage Research, 2018, 177: 79.
