Abstract：The reaction of hemoglobin (Hb) with copper(Ⅱ)-Alizarin red S (ARS) complex was studied in H3PO4-KH2PO4 buffer solution (pH 4.2) by ultraviolet-visible spectrophotometry. The results show that the interaction of Hb and Cu(Ⅱ)-ARS complex produces red ionic association complex with its maximum absorption peak at 537 nm. At the maximum absorption, the composition of the complex was determined to be nHb∶nCu(Ⅱ)∶nARS=1∶4∶8, and the apparent molar absorptivity was 1.52×105 L·mol-1·cm-1. The concentration of Hb is linear with the absorbency in the range of 1.0×10-7-2.0×10-6 mol·L-1 and the regression equation was established as A=0.026 9+151 675ｃ(mol·L-1) with the coefficient r=0.997 2. The effects of solution acidity, reagent amount, reaction time, temperature, ionic strength and the added surfactant were examined on the formation of the Hb-Cu(Ⅱ)-ARS complex. A preliminary investigation was carried out to elucidate the reaction mechanism, and it could be concluded that the Hb and Cu(Ⅱ)-ARS complex are combined mainly by electrostatic attraction. Further investigation was also undertaken to find out the effects of common amino acids and metallic ions on the formation of Hb-Cu(Ⅱ)-ARS complex.
Key words：Hemoglobin;Copper(Ⅱ)-alizarin red S complex;Ultraviolet-visible spectrophotometry
吴小华,缪吉根,缪煜清,陈建荣. 血红蛋白与铜(Ⅱ)-茜素红S络合物相互作用的研究[J]. 光谱学与光谱分析, 2007, 27(06): 1168-1171.
WU Xiao-hua,MIAO Ji-gen,MIAO Yu-qing,CHEN Jian-rong. Study on the Interaction of Hemoglobin and Cu(Ⅱ)-ARS Complex. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(06): 1168-1171.
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