Study on RBC Oxygen-Carrying Function with the Incubation Time
LUO Man1*, HUANG Yuan-yuan1, SU Bao-chang2, SHI Yun-feng3, ZHANG Hong1, YE Xun-da1
1. Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China 2. Department of Blood Transfusion, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China 3.Center of Experimental Technology, Jinan University, Guangzhou 510632, China
Abstract:The cycle of Hemoglobin oxygenation and deoxidation plays an important role in driving structure and regulating function of red blood cell in vivo, has attracted wide attention. But it has not yet been reported about any studies on the oxygen-carrying function of individual living RBC in vitro with the incubation time. This study involved that using confocal Raman scanning microscopic technique, collecting the Raman spectra of living erythrocyte cultured in vitro at different time, analysing the peaks (1 636, 1 562 cm-1) with characterization of hemoglobin oxygen carrying capacity and the amide Ⅲ band (1 240~1 300 cm-1) with sensitivity to conformation for understanding those changes both of hemoglobin oxygen carrying capacity and protein conformation over time. Meanwhile, its corresponding surface micromorphology was observed via scanning electron microscopy (SEM). The results indicated that the during 24 h hemoglobin with stable structure and normal allosteric collaborative function occurred alternately with the oxygen uptake of increase and decrease and conformation R state and T state, while double concave disk red blood cells observed under SEM also alternately between stretch and shrink. The conclusion not only provides a multi-level characteristic parameter from a single living cell for the study of RBC oxygen-carrying function in vitro, but also the potential research ideas for the screening of the active component, the evaluation of drug efficacy and toxicity for RBC in vitro.
罗 曼1*,黄媛媛1,苏宝倡2,施云峰3,张 红1,叶迅达1 . 红细胞携氧功能随孵育时间动态变化的研究 [J]. 光谱学与光谱分析, 2015, 35(12): 3350-3355.
LUO Man1*, HUANG Yuan-yuan1, SU Bao-chang2, SHI Yun-feng3, ZHANG Hong1, YE Xun-da1 . Study on RBC Oxygen-Carrying Function with the Incubation Time . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(12): 3350-3355.
[1] Perutz M F. Molecular Anatomy, Physiology and Pathology of Hemoglobin. In: Molecular Basis of Blood Diseases. Philadelphia, W B Saunders Co, 1987, 127. [2] Maria Crist ina De Rosa, Cristia na Carelli, Ali novi, et al. Iubmb Life, 2008, 60(2): 87. [3] Phillips W T, Lemen L, Goins B, et al. The American Journal of Physiology, 1997, 5 (27) : 2492. [4] LI Yao-jin, WANG Xiang, LAN Ke(李遥金,王 翔,兰 珂). China J Blood Transfusion (中国输血杂志), 2009, 6(22): 439. [5] Yang Rongwu, Gao Ge, Li Genxin, et al. Electrochemistry Communications,2007,9: 94. [6] Ramser K, Logg K, Goksor M. J. Biomed. Opt., 2004, 9(3): 593. [7] Brazhe, Nadezda A, Abdali Salim, et al. Biophysical Journal, 2009, 97: 3206. [8] Thomas G Spiro, Thomas C Strekas. Proc. Natl Acad. Sci., USA, 1972, 69(9): 2622. [9] Thomas G Spiro, Thomas C Strekas. Journal of the American Chemical Society, 1974, 2(96): 338. [10] CAO Shui-liang,ZHU Yuan-zong,GUO Zu-wen(曹水良,朱元宗,郭祖文). Journal of Jinan University(暨南大学学报),2013,3(34):315. [11] XU Yi-ming(许以明). Raman Spectroscopy in Application of Structure Biology(拉曼光谱及其在结构生物学中的应用). Beijing: Chemical Industry Press(北京:化学工业出版社), 2005, 11. [12] Bayden R Wood, Brian Tait, McNaughton D. Biochimica et Biophysica Acta, 2001, 1539: 58. [13] Nadezda A Brazhe, Salim Abdali, Alexey R Brazhe, et al. Biophysical Journal,2009, 12(97): 3206. [14] Wood B R, Caspers P, Puppels G J, et al. Anal. Bioanal. Chem., 2007, 387: 1691. [15] Bayden R Wood, Don McNaughton. J. Raman Spectrosc., 2002, 33: 517. [16] Bayden R Wood, Larissa Hammer, Lara Davis, et al. Journal of Biomedical Optics, 2005, 10(1): 014005. [17] Messana I, Orlando M, Cassiano L, et al. FEBS Lett., 1996, 390: 25. [18] Low P S, Rathinavelu P, Harrison M L. J. Biol. Chem., 1993, 268: 14627. [19] Walder J A, Chatterjee R, Steck T L, et al. J. Biol. Chem., 1984, 259: 10238. [20] Salhany J M, Cassoly R J. Biol. Chem., 1989, 264: 1399. [21] Andrew J Gow, Jonathan S StamLer. Nature, 1998, 6663(391): 169. [22] Gugliotta Tiziana, De Luca Grazia, Romano Pietro, et al. Pharmacology & Pharmacy, 2011, 2: 116. [23] Moriyama R, Lombardo C R, Workman, et al. J. Biol. Chem., 1993, 268: 10990.