Study on Multispectral Polarization Characteristics of Biological Tissues
QIAO Wen-long1, 2, ZHOU Liang1*, LIU Zhao-hui1, GONG Yong-hui3, JIANG Le1, LÜ Yuan-yuan1, 2, ZHAO He-tong1, 2
1. Xi’an Institute of Optics and Precision Mechanics of Chinese Academy of Sciences, Xi’an 710119, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
3. Unit 63636 of Jiuquan Satellite Launch Center, Jiuquan 732750, China
Abstract：Biological tissues are very complicated with strong scattering characteristics. The light-source of detecting physiological parameters of tissues is critical. Combined with the advantages of polarization imaging, this paper studies the multispectral polarization characteristics of biological tissues. We established uniform monolayer biological tissue samples based on the distribution of different particle sizes and simulated the scattering model with single-particle by combining Rayleigh and Mie scattering theory. Rayleigh theory has good forward and backward scattering symmetry; Mie theory has strong forward scattering characteristics. The two scattering models are closely related to the size parameter, a dimensionless quantity, depending on the incident wavelength and the size of scattered particles. Mie theory is generally used as the research model in biological tissues. We used a Monte Carlo method to simulate the transmission characteristics of polarized light in the tissue model. The wavelength range is 400~1 000 nm. In this paper, we have simulated four typical polarization states, (horizontally polarized light, vertically polarized light, 450 linearly polarized light and right-rotated circularly polarized light). The experiment system used a white LED lamp as a light source. It used filters to obtain different wavelength beams, a color camera was used to record the image of the target, two groups of linear polarizers and right-rotated circular polarizers were used as polarizers and analyzers to test horizontally polarized light, and right-rotated circular polarized light with wavelengths of 450, 525, 550, 590, 610, 650 and 690 nm, respectively, and the target is our palm. Both simulation and experimental results show that with the increase of wavelength, the degree of polarization (DOP) of linearly polarized light after backscattering from skin tissue shows an overall upward trend, while that of circularly polarized light is on the decline. However, the overall DOP of circularly polarized light is higher than that of linearly polarized light, which indicates that circularly polarized light has better polarization retention than linearly polarized light in biological tissues and is more suitable for detecting physiological information. Our study has confirmed the multispectral characteristics of circularly polarized light and linearly polarized light transmitted in tissues, which provided theoretical support for obtaining multispectral polarization physiological parameters.
[1] Ushenko V, Sdobnov A, Syvokorovskaya A, et al. Photonics，2018，5(4): 54.
[2] Zaffar M，Pradhan A. Appl. Opt.，2020，59(4)：1237.
[3] Borovkova M, Peyvasteh M, Ushenko Y, et al. J. Eur. Opt. Soc. Rapid Publ.，2018，14：20.
[4] Valery V Tuchin. J. Biomed. Opt.，2016，21(7)：071114.
[5] Zhang Yong, Chen Bin, Li Dong. Appl. Opt., 2016, 55: 2681.
[6] Glaser A K, Chen Y, Liu J T C，et al. Optica, 2016, 3: 861.
[7] Otsuki S. Appl. Opt., 2016, 55: 5652.
[8] Shen Z, Sukhov S, Dogariu A. J. Opt. Soc. Am. A，2017，34, 2189.
[9] Periyasamy V，Pramanik M. IEEE Reviews in Biomedical Engineering, 2017，10：122.
[10] ZHOU Liang, YU Jiang-jun, LIU Zhao-hui, et al（周 亮，余江军，刘朝晖，等）. Spectroscopy and Spectral Analysis（光谱学与光谱分析），2020，40(4): 1071.
[11] Nishizawa N, Hamada A, Takahashi K. Japanese Journal of Applied Physics 2020，59：1347.
[12] WANG Li-hong, WU Xin-yi(汪立宏，吴新一). Biomedical Optics Principles and Imaging(生物医学光学原理和成像). Hefei: University of Science and Technology of China Press(合肥：中国科学技术大学出版社)，2017. 7.
