Fast Measurement of Primary Productivity in the Yellow Sea and Bohai Sea Based on Fluorescence Kinetics Technology
WANG Xiang1, 2, YIN Gao-fang1*, ZHAO Nan-jing1, GAN Ting-ting1, YANG Rui-fang1, QIN Zhi-song3, DONG Ming1, 2, CHEN Min1, 2, DING Zhi-chao1, 2, QI Pei-long1, 2, WANG Lu1, 2, MA Ming-jun1, 2, MENG De-shuo1, LIU Jian-guo1
1. Key Laboratory of Environment Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China
2. University of Science and Technology of China, Hefei 230026, China
3. Institute of Computer and Information Security, Guilin University of Electronic Technology, Guilin 541004, China
Abstract：As an important -starting point in the marine ecosystem, phytoplankton primary production is the foundation of the marine food web and an important indicator for seawater quality. In this paper, in order to accurately measure, monitor, and predict spatiotemporal variations of phytoplankton primary productivity, and its kinetic response to external environmental conditions, a measurement technology based on chlorophyll fluorescence kinetics was studied, and a fast measuring instrument with a response time of 1.6 minutes and a precision of 4.86% was developed. The correlation coefficient between the measured phytoplankton primary productivity by the developed instrument and the measured photosynthetic oxygen evolution rate by the liquid-phase oxygen electrode was 0.991. The instrument was equipped on the China Marine Surveillance 101 experimental ship for the underway measurement and the vertical profiles measurement of phytoplankton primary productivity in the Yellow Sea and the Bohai Sea. Underway measurement results showed that the phytoplankton primary productivity in Bohai bay is 1.1~1.4 times higher than outside the bay, and vertical profiles measurement results showed a depth-dependent response of phytoplankton primary productivity on each site in the Yellow sea area. Compared with traditional methods, the developed instrument in this paper has the advantages of rapidness, stability, and no need for sample cultivation and can provide an advanced technical method for marine ecological environment assessment.
基金资助: The National Key Research and Development Plan in China (2021YFC3200100, 2016YFC1400600)，the National Natural Science Foundation of China (61875207，61805254)，the Anhui provincial Excellent Youth Science Foundation of China (1908085J23)，Plan for Major Provincial Science&Technology Project (202003a07020007)，Technological Innovation Project of Instrument and Equipment Function Development of Chinese Academy of Sciences (Y93H3g1251), Anhui Polytechnic University Research Startup Foundation (2021YQQ034), The Open Research Fund of Anhui Province Key Laboratory of Detection Technology and Energy Saving Devices, Anhui Polytechnic University (JCKJ2021B01)
通讯作者:
殷高方
E-mail: gfyin@aiofm.ac.cn
作者简介: WANG Xiang, (1988—), Ph.D. candidate, mainly focuses on fluorescence detection and analysis technology
e-mail: xwang@aiofm.ac.cn
引用本文:
王 翔，殷高方，赵南京，甘婷婷，杨瑞芳，覃志松，董 鸣，陈 敏，丁志超，亓培龙，王 璐，马明俊，孟德硕，刘建国. 基于荧光动力学技术的黄渤海初级生产力快速测量[J]. 光谱学与光谱分析, 2022, 42(03): 990-996.
WANG Xiang, YIN Gao-fang, ZHAO Nan-jing, GAN Ting-ting, YANG Rui-fang, QIN Zhi-song, DONG Ming, CHEN Min, DING Zhi-chao, QI Pei-long, WANG Lu, MA Ming-jun, MENG De-shuo, LIU Jian-guo. Fast Measurement of Primary Productivity in the Yellow Sea and Bohai Sea Based on Fluorescence Kinetics Technology. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 990-996.
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