1. 医学光电科学与技术教育部重点实验室，福建师范大学, 福建 福州 350007 2. 福建省肿瘤医院，福建 福州 350001 3. Cancer Imaging Department, BC Cancer Agency, Vancouver, Canada V5Z 1L3
Measurement of Nasopharyngeal Carcinoma Tissue ex vivo by Raman Spectroscopy
HUANG Wei1, PAN Jian-ji2, CHEN Rong1*, LI Yong-zeng1, FENG Shang-yuan1, XIE Shu-sen1, ZENG Hai-shan3
1. Key Laboratory of Optoelectronic Science and Technology for Medicine ( Fujian Normal University ), Ministry of Education, Fuzhou 350007, China 2. Fujian Provincial Tumor Hospital, Fuzhou 350001，China 3. Cancer Imaging Department, BC Cancer Agency, Vancouver, Canada V5Z 1L3
Abstract：Raman spectroscopy has shown its potential and advantages in detecting molecular changes associated with tissue pathology, which makes it possible to diagnose with optical methods non-invasively and real-time. A compact and rapid near-infrared (NIR)Raman system was developed using 785 nm diode laser, volume phase technology (VPT)holographic grating system and NIR intensified charge-coupled device (CCD)with a specially designed Raman fibre probe which can effectively reduce the interference of fluorescence and Rayleigh scattering, maximize the ability of Raman collection as well as correct the image aberration of a planar grating diffraction. Adopting this method, signal-to-noise ratio has been greatly improved and human tissue signals can be acquired in a short time. Raman signals from fat and musculature of fresh pork were measured and referenced for further optimization, then Raman spectra of nasopharyngeal carcinoma in vitro and the effect of storage time on them were measured in 1-5 s and discussed. The sensitivities and performance of the system will be further enhanced and more Raman data will be acquired and compared between normal and cancerous nasopharyngeal tissue, expecting to discover the statistical characteristics, which will benefit the diagnosis and treatment of early nasopharyngeal carcinoma or other tumors.
Key words：Raman spectroscopy;Nasopharyngeal carcinoma;Tissue ex vivo;Rapid detection
