Abstract：In the present paper，improved partial least squares(PLS) method was used to parse near infrared(NIR) reflectance spectrum of Cofrel medicine. The contents of benproperine phosphate，a availability pharmaceutical in Cofrel medicine，were accurately nondestructively quantitatively predicted. The best number of principal components (PC) was designed by scores plot of samples in PC. The effects of the wavelength range，conventional spectra，first-derivative spectra and second-derivative spectra on the results were discussed respectively. Comparing the results with those of HPLC，the relative errors(RE%) of benproperine phosphate were less than 0.42%. The analytical results were sufficient for practical manufacture of Cofrel medicine.
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