光谱学与光谱分析
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Triton X-100微乳液中铈与L-色氨酸荧光反应的研究与应用
龚爱琴1,2 ,朱霞石1* ,郭荣1 ,马晓芹1
1. 扬州大学化学化工学院, 江苏 扬州 225002 2. 扬州工业职业技术学院,江苏 扬州 225002
Investigation and Application of Fluorescence Reaction of Cerium-L-Tryptophan in Triton X-100 Microemulsion
GONG Ai-qin1,2 ,ZHU Xia-shi1* ,GUO Rong1 ,MA Xiao-qin1
1. Department of Chemistry, Yangzhou University, Yangzhou 225002, China 2. Yangzhou Polytechnic Institute, Yangzhou 225002, China
摘要 : 研究了Triton X-100微乳液(Triton X-100-M)对荧光法测定L-色氨酸的增敏作用。在酸性条件中Ce4+ 可将色氨酸氧化为强荧光性物质,以此为基础建立了分析色氨酸的新方法。与Triton X-100胶束相比,Triton X-100微乳液对荧光法测定L-色氨酸具有更好的增敏作用,在该介质中测定灵敏度提高了225倍。实验中对影响荧光测定的各种因素进行了优化,同时考察了共存离子的干扰并做了回收试验,对荧光增敏机理也进行了初步探讨。试验结果表明:在优化条件下,色氨酸浓度在0.1~1.0 μg·mL-1 范围内与荧光强度成线形关系,其线性方程为F =342.37+30.45c ,r =0.998 4,检出限为0.09 μg·mL-1 。在优化参数条件下,对实际样品进行分析,结果令人满意。
关键词 :L-色氨酸;荧光分析;微乳液;增敏作用
Abstract :In the present article, the sensitizing effect of Triton X-100 microemulsion media (Triton X-100-M) on the determination of L-tryptophan by fluorescence spectrophotometry was studied, which was based on a strong fluorescence substance produced when L-tryptophan was oxidized by Ce4+ in acid medium. Compared with Triton X-100 micelle (Triton X-100-m), it was found that in the medium of Triton X-100-M the intensity of fluorescence was enhanced by 225 times. Various factors that influence fluorescence determination and interfering ions were examined. The mechanism of sensitizing effect of Triton X-100 microemulsion was also discussed. Under the optimum conditions, the liner range and detection limit are 0.1-1.0 μg·mL-1 and 0.09 μg·mL-1 (correlation coefficient r =0.998 4), respectively. The linear equation was F =342.37+30.45c . The proposed method has been successfully applied to determine food samples.
Key words :L-tryptophan;Fluorescence;Microemulsion;Sensitizing effect
收稿日期: 2006-10-29
修订日期: 2007-02-02
通讯作者:
朱霞石
E-mail: xszhu@yzu.edu.cn
引用本文:
龚爱琴1,2 ,朱霞石1* ,郭荣1 ,马晓芹1 . Triton X-100微乳液中铈与L-色氨酸荧光反应的研究与应用[J]. 光谱学与光谱分析, 2008, 28(04): 900-903.
GONG Ai-qin1,2 ,ZHU Xia-shi1* ,GUO Rong1 ,MA Xiao-qin1 . Investigation and Application of Fluorescence Reaction of Cerium-L-Tryptophan in Triton X-100 Microemulsion. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(04): 900-903.
链接本文:
https://www.gpxygpfx.com/CN/10.3964/j.issn.1000-0593.2008.04.015
或
https://www.gpxygpfx.com/CN/Y2008/V28/I04/900
[1] Jason W Costin, Paul S Francis, Simon W Lewis. Anal. Chim. Acta, 2003, 480: 67. [2] WANG Jun, SUN Guang-ming, WANG Li, et al(汪 俊, 孙光明, 王 利, 等). Chinese J. Anal. Chem.(分析化学), 2006, 34(2): 267. [3] WANG Li-sheng, PENG Zhong-li, JIANG Zhi-liang(王力生, 彭忠利, 蒋治良, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(4): 641. [4] DING Ya-yun, XIE Meng-xia, KANG Juan(丁雅韵, 谢孟峡, 康 娟). Chinese J. Anal. Chem.(分析化学), 2002, 30(4): 418. [5] WU Jian-min, SHI Qing-zhao, WANG Guo-shun, et al(邬建敏, 施清照, 王国顺, 等). Chinese J. Anal. Chem.(分析化学), 1994, 22(6): 599. [6] YANG Li-li, YUAN Zhuo-bin(杨莉丽, 袁倬斌). Chinese J. Anal. Chem.(分析化学), 1999, 27(9): 1065. [7] Qu Qishu, Tang Xiaoqin, Hu Xiaoya. Anal. Chim. Acta, 2006, 572: 212. [8] SUN Yan-hua, YANG Jing-zhi, ZHOU Jie, et al(孙衍华, 杨景芝, 周 杰, 等). Chinese J. Anal. Chem.(分析化学), 2004, 32(8): 1067. [9] ZHU Xia-shi, GUO Rong, QI Wen-bin(朱霞石, 郭 荣, 戚文彬). Chinese J. Anal. Chem.(分析化学), 1995, 23(9): 989. [10] ZHU Xia-shi, GUO Rong(朱霞石, 郭 荣). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1999, 19(3): 456. [11] ZHU Xia-shi, GUO Rong, KANG Chun(朱霞石, 郭 荣, 康 纯, 等). Acta Chimica Sinica(化学学报), 1995, 53:716. [12] ZHU Xia-shi, GUO Rong, LIU Xiu-mei(朱霞石, 郭 荣, 刘秀梅). Chinese J. Anal. Chem.(分析化学), 1994, 22(8): 860. [13] ZHU Xia-shi, GUO Rong, ZHANG Xia-hong(朱霞石, 郭 荣, 张晓红). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(4): 515. [14] Zhu X S, Bao L, Guo R et al. Anal. Chim. Acta, 2004, 523: 43. [15] ZHU Xia-shi, GUO Rong(朱霞石, 郭 荣). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(2): 314.
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