Abstract：Based on the complex formed by Zn2+ which can strengthen the relative fluorescence intensity of fleroxacin evidently, a novel photochemical fluorescence method was developed. The effects of the acidity, the concentration ratio of Zn2+ to fleroxacin，and the time for illumination were studied. Under the optimum experiment conditions, the linear range of the determination was 5.00×10-8-5.00×10-6 mol·L-1. The detection limit was 4.2×10-8 mol·L-1. The relative standard deviation of the determination of fleroxacin (5.0×10-7 mol·L-1) was 1.7% (n=20). The method was successfully applied to the determination of fleroxacin in specimens, and the recoveries were in the range of 95.0%-105%. The mechanism of this system is proposed.
[1] Morin M. Anal. Chim. Acta, 1989, 219: 67. [2] Predrag Djurdjevic, Milena Jelikic-Stankov, Aleksandra Laban. Talanta，2001, 55: 631. [3] Eva M Golet, Alfredo C Alder, Andres Hartmann，et al. Anal. Chem.，2002, 73: 3632. [4] DU Li-ming，FAN Zhe-feng，ZHANG Rui-feng(杜黎明，范哲锋，张瑞凤). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003, 23(2)：328. [5] Lydia J Martinez, Galaxy Li，Colin F Chignell. Photochemistry and Photobiology, 1997, 65: 599. [6] ZHANG Tie-li, ZHAO Hui-chun, Jin Lin-pei，et al. Journal of Photochemistry and Photobiology A：Chemistry，1999, 121: 37. [7] LI Hua，et al(李 华，等). Chinese Journal of New Drugs(中国新药杂志)，1996, 5(1): 55.
