Application and Development of Spectroscopy Methodologies in the Study on Non-Covalent Interactions
LI Rui1,DAI Ben-cai1,ZHAO Yong-de1*,LU Kui2
1. Henan Institite of Chemistry, Henan Academy of Sciences, Zhengzhou 450002, China 2. School of Chemistry and Chemical Engineering,Henan University of Technology,Zhengzhou 450001,China
Abstract:Spectrophotometric method is widely used in the structure determination of biologic macromolecules and non-covalent interactions study for its convenience and speed. In the present paper, spectroscopy methodologies in the study of non-covalent interactions between small-molecule and biomacromolecule is comprehensively reviewed with 25 references.This review article focuses on the applications and development of common spectroscopy methodologies in the study of non-covalent interactions between small molecule and biomacromolecule,including the UV, fluorescence, CD, IR, Raman, resonance light scattering technique and SPR. The advantages and disadvantages of spectroscopy methodologies are also described. UV-Vis absorption spectrum (UV) method is widely used in the study of non-covalent interactions for its convenience and speed. The binding site number, the apparent binding constant and the interaction mode of non-covalent interactions can be obtained by fluorescence spectrum method. Circular dichroism (CD) method is effective way in the study of non-covalent interactions measure. Spectroscopy information about protein secondary structure and conformation can be acquired by infrared spectrometry (IR) method. Raman spectroscopy method is a better way to investigate the conformation change in macromolecules in solution. Non-covalent interactions can be measured by surface plasma resonance (SPR) method under the natural active condition. X-ray diffraction analysis method is better for non-covalent interactions research, but it is difficult to cultivate crystalline complex.
李锐1,代本才1,赵永德1*,卢奎2 . 光谱法在分子间非共价相互作用中的应用及进展[J]. 光谱学与光谱分析, 2009, 29(01): 240-243.
LI Rui1,DAI Ben-cai1,ZHAO Yong-de1*,LU Kui2. Application and Development of Spectroscopy Methodologies in the Study on Non-Covalent Interactions. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(01): 240-243.
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