1. College of Sciences, Hebei University of Science and Technology, Shijiazhuang 050018, China 2. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China 3. Key Laboratory of Analytical Sciences of MOE and Department of Chemistry, Xiamen University, Xiamen 361005, China
Abstract：Taking tetramethoxysilane (TMOS) and dimethyldimethoxysilane (DiMe-DMOS) as precursors for the synthesis of an organically-modified silicates (ORMOSILs)，optical BOD sensing films immobilizing five kinds of limnetic microorganisms were composed by poly(vinyl alcohol) ormosils matrix. Their fluorescence characteristics were studied with a home-made optical BOD apparatus. According to the experimental results, linearity between BOD and the corresponding fluorescence intensity ranging from 0-60 mg·L-1 to 0-120 mg·L-1 could be obtained on the sensing films immobilizing limnetic microorganisms and their mixture. In addition, the linearity coefficient was from 0.976 to 0.997, and the respond time was 0.5 to 8.7 min. In the experiment, effects of the measurement condition such as temperature and pH on the fluorescent responses of BOD sensing films were investigated. These sensing films presented excellent reproducibility and stability since they could be continuously employed for 30 days, and kept 85% of their original activity when stored for 12 months at 4 ℃. The approved approach could be applied in the BOD determination of real limnetic samples according to the obtained results.
郭光美1,3,辛玲玲3,王旭东3,赵赟3,陈曦2,3*. 包埋不同淡水耗氧菌BOD传感膜的光谱响应特征研究[J]. 光谱学与光谱分析, 2008, 28(09): 2134-2138.
GUO Guang-mei1,3,XIN Ling-ling3,WANG Xu-dong3,ZHAO Yun3,CHEN Xi2,3*. Study on the Fluorescence Characteristics of BOD Sensing Films Immobilizing Different Limnetic Microorganism. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(09): 2134-2138.
[1] ZHAO Li, XIAO Lai-long, LIN Ling, et al(赵 丽，肖来龙，林 玲, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(12)：2039. [2] Yang Z，Sasaki S，Karube I. Analytica Chimica Acta，1997，357：4. [3] LIN Ling, DAI Yuan-jing, XIAO Lai-long, et al(林 玲，戴媛静，肖来龙, 等). Ocean Technology(海洋技术)，2004，23：53. [4] Jianbo J，Mingyu T，Xu Ch, et al. Biosensors & Bioelectronics，2003，18：1023. [5] Ngayan K，Shaojun D，Waihung L, et al. Sensors and Actuators B，2005，110：289. [6] Gabjoo Ch，Yoko N，Isao K. Analytica Chimica Acta，1999，379：185. [7] LI Hua-zi, SHI Han-chang, WANG Jian-long, et al(李花子，施汉昌，王建龙, 等). Chinese High Technology Letters(高技术通讯)，2002，7：93. [8] Liu J，Bjornsson L，Mattiasson B. Biosensors & Bioelectronics, 2000，14：883.
