核酸-桑色素-铝(Ⅲ)三元荧光体系的研究

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摘要：基于核酸对桑色素-铝(Ⅲ)配合物的荧光增强作用，以桑色素-铝(Ⅲ)为荧光探针，考察该探针与核酸的结合反应，建立了新的准确测定核酸的方法，并研究了该三元荧光体系的作用机理。在pH 8.5时，fsDNA，ctDNA，smDNA和yRNA的浓度与桑色素-铝(Ⅲ)的荧光强度成线性关系，响应范围分别为0.25～1.50，0.25～2.00，0.10～1.60和0.25～2.00 μg·mL^-1，检测限(3σ/K)分别为3，2，2和3 ng·mL^-1。测定了合成样品，回收率93.3%～107.9%，相对标准偏差小于3.6%。

关键词：桑色素-铝(Ⅲ);核酸;荧光探针

Abstract：A fluorescence-enhanced system was developed for the determination of nucleic acids by using morin-aluminum(Ⅲ) complex as a new fluorescent probe. In aqueous solution, morin-aluminum(Ⅲ) complex showed maximum excitation and emission wavelengths at 420.0 nm and 532.8 nm, respectively, and its fluorescence could be greatly enhanced in the presence of nucleic acids. Under optimal conditions, the calibration graph was linear over the range 0.25-1.50 μg·mL^-1 for fish sperm DNA, 0.10-1.60 μg·mL^-1 for salmon sperm DNA, 0.25-2.00 μg·mL^-1 for calf thymus DNA and 0.25-2.00 μg·mL^-1 for yeast RNA. The corresponding detection limits are 3 ng·mL^-1, 2 ng·mL^-1, 2 ng·mL^-1 and 3 ng·mL^-1, respectively. Applied for the determination of nucleic acids in synthetic samples, the relative standard deviation for five replicates is less than 3.6%, and the recovery ranges from 93.3% to 107.9%. Additionally, the interaction mechanism of morin-aluminum(Ⅲ) with nucleic acids is also discussed.

Key words：Morin-aluminum(Ⅲ);Nucleic acids;Fluorescent robe

收稿日期: 2003-04-06 修订日期: 2004-08-18

中图分类号:

O657.32

通讯作者: 谢增鸿

引用本文:

林旭聪, 谢增鸿^*, 郭良洽, 陈国南 . 核酸-桑色素-铝(Ⅲ)三元荧光体系的研究 [J]. 光谱学与光谱分析, 2004, 24(10): 1230-1234.
LIN Xu-cong, XIE Zeng-hong^*, GUO Liang-qia, CHEN Guo-nan . Studies on a New Fluorescence-Enhanced System of Nucleic Acids-Morin-Al(Ⅲ) . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(10): 1230-1234.

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http://www.gpxygpfx.com/CN/Y2004/V24/I10/1230