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| Preparation, Spectral Properties and Antioxidant Activities of Pyranone-Anthocyanin Derivative (Oxovitisin) |
| WU Nao, WANG Jing-yi, JIANG Tian, LI Shu-yi, ZHU Zhen-zhou, HE Jing-ren* |
| Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China |
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Abstract A novel polyphenolic compound, named Oxovitisin, with unique lactone pyranone ring and non-oxonium structures was prepared. In this paper, Oxovitisin A was synthesized with two-step reactions in micro-oxygenation system using carboxypyranoanthocyanins formed from pyruvic acid and anthocyanins. The fraction of Oxovitisin A was isolated by a combination of several chromatographic techniques and identified with HPLC-DAD-EI-MS/MS. The results showed that the purity of Oxovitisin A could reach more than 99% after purification. The study investigated the florescence spectra of Oxovitisin A, the effect of different pH value on UV feature and color parameters. The antioxidant activities of Oxovitisin A were evaluated by using a BPCL Ultra-weak luminescence analyzer in vitro, which were compared with that of Vitisin A, Mv-3-gluc and Vitamin C. The results revealed that Oxovitisin A had an obvious fluorescence peaks under the excitation wavelength of 440 nm, but no intensity of florescence was detected on Vitisin A and Mv-3-gluc. Analysis with UV spectroscopy and color parameter variations method indicated that Oxovitisin A had a higher structural and color stability. All four compounds (Oxovitisin A, Vitisin A, Mv-3-gluc, Vitamin C) showed strong antioxidant activity in scavenging O-2 (IC50 of 71.4, 30.7, 19, 28 μg·mL-1, respectively), ·OH (1.68, 3.524, 2.854, 8.441 μg·mL-1, respectively) and H2O2 (1.311, 0.409 8, 0.288, 3.265 μg·mL-1, respectively). However, Oxovitisin A showed the highest antioxidant ability in scavenging free radical compared with Vitisin A, Mv-3-gluc and Vitamin C.
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Received: 2015-11-17
Accepted: 2016-05-10
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Corresponding Authors:
HE Jing-ren
E-mail: jingren.he@whpu.edu.cn
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