| 光谱学与光谱分析 |
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| Study on BS-12 as a New Probe for the Determination of DNA by Resonance Light Scattering |
| CHEN Zhan-guang1, 2, LIAO Xu-hong1, HAN Ya-li1, YANG Ying-yi1, LU-Feng1, DING Wei-feng1 |
1. Science College, Shantou University, Shantou 515063, China
2. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China |
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Abstract A new method has been developed for the determination of DNA by resonance light scattering with dodecyl dimethyl betaine (BS-12) in aqueous solution as a new probe. At pH 9.3, the interactions of BS-12 and DNA gave strong RLS signals at 388.0 nm. Linear relationships were found between the enhanced intensity of RLS and the concentration of DNAs in the range 0.25-12.0 μg·mL-1for fsDNA and 0.25-11.0 μg·mL-1 for ctDNA. The limits of detection were 0.15 ng·mL-1 and 0.16 ng·mL-1 for fsDNA and ctDNA, respectively. The method was applied to the determination of synthetic samples with satisfactory results.
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Received: 2004-06-29
Accepted: 2004-09-28
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Corresponding Authors:
CHEN Zhan-guang
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| Cite this article: |
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CHEN Zhan-guang,LIAO Xu-hong,HAN Ya-li, et al. Study on BS-12 as a New Probe for the Determination of DNA by Resonance Light Scattering[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(10): 1680-1683.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I10/1680 |
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