采用氧合血红蛋白吸收带的漫反射光谱比率R540/R575检测浅表性膀胱癌

doi:10.3964/j.issn.1000-0593(2008)11-2721-05

Abstract
References
Related Citation (15)

Download: PDF (682 KB)
Export: BibTeX | EndNote (RIS)

Abstract A low-cost, fast, and noninvasive method for early diagnosis of malignant lesions of mucosa tissue based on diffuse reflectance spectra was applied in the study of the optical biopsy of superficial human bladder cancer. In the present paper, differential diagnosis of superficial human bladder cancer was studied using the diffuse reflectance spectral ratio (R₅₄₀/R₅₇₅) of the oxygenated hemoglobin absorption bands at 540 and 575 nm in vitro. Diffuse reflectance spectra for mucosa/submucosa tissues of normal bladder and superficial bladder cancer were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that there were three the diffuse reflectance spectral dips at 415, 542 and 577 nm respectively for mucosa/submucosa tissues of normal bladder and superficial bladder cancer in the spectral range from 400 to 600 nm. The mean diffuse reflectance spectral ratio (R₅₄₀/R₅₇₅) of normal bladder mucosa/submucosa tissue decreased slowly with time increase after surgical excision, and the mean diffuse reflectance spectral ratio (R₅₄₀/R₅₇₅) of superficial bladder cancer mucosa/submucosa tissue also decreased slowly with time increasing after surgical excision. The mean diffuse reflectance spectral ratios (R₅₄₀/R₅₇₅) of normal bladder mucosa/submucosa tissue were 111%, 107%, 104% and 102% after 2, 3, 4 and 5 h after surgical excision respectively, and those of superficial bladder cancer mucosa/submucosa tissue were 98.4%, 95.5%, 93.1% and 91.6% after 2, 3, 4 and 5 h after surgical excision respectively. There were significant differences in mean diffuse reflectance spectral ratio (R₅₄₀/R₅₇₅) for mucosa/submucosa tissues between normal bladder and superficial bladder cancer after 2, 3, 4 and 5 h after surgical excision respectively(p＜0.05). Differences in mean diffuse reflectance spectral ratio (R₅₄₀/R₅₇₅) for mucosa/submucosa tissues between normal bladder and superficial bladder cancer were 12.6%, 11.5%, 10.9% and 10.4% after 2, 3, 4 and 5 h after surgical excision respectively. It is obvious that pathological changes in bladder mucosa/submucosa tissues induced changes in the component and structure of the tissues, and especially quantitative changes in oxyhemoglobin and de-oxyhemoglobin of tissues obviously. Conclusion of the study provides a new method that can be applied to rapid, low-cost and noninvasive optical biopsy of superficial bladder cancer.

Key words：Superficial bladder cancer diagnosis;Diffuse reflectance spectroscopy;Diffuse reflectance spectral ratio (R₅₄₀/R₅₇₅);Hemoglobin absorption peaks

Received: 2007-09-08 Accepted: 2007-12-18

ZTFLH:

R73-3

Corresponding Authors: XING Da E-mail: xingda@scnu.edu.cn

Cite this article:

WEI Hua-jiang,XING Da,WU Guo-yong, et al. Superficial Bladder Cancer Detection Using Diffuse Reflectance Spectral Ratio R₅₄₀/R₅₇₅ of Oxygenated Hemoglobin Bands[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(11): 2721-2725.

URL:

https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2008)11-2721-05 OR https://www.gpxygpfx.com/EN/Y2008/V28/I11/2721

[1] Mourant J R, Bigio I J, Boyer J, et al. Lasers Surg. Med., 1995, 17: 350.
[2] Ge Z, Schomacker K T, Nishioka N S. Appl. Spectrosc., 1998, 52: 833.
[3] Feld M S. Technical Digest, 1998, 2: 1.
[4] Zonios G, Perelmann L T, Bacjmann V, et al. Appl. Opt., 1999, 38: 6628.
[5] Sokolov K, Drezek R, Gossage K, et al. Opt. Express. 1999, 5: 302.
[6] Utzinger U, Brewer M, Silva E, et al. Lasers Surg. Med., 2001, 28: 56.
[7] Zijlstra W G, Buursma A, Meeuwsen-van der Roest W P. Clinical. Chem., 1991, 37(9): 1633.
[8] Crawford E D, Wood D P, Petrylak D P, et al. Cancer, 2003, 97(8 Suppl): 2099.
[9] Kim Y L, Liu Y, Wali R K, et al. IEEE J. Select. Topics Quantum Electronics, 2003, 9: 243.
[10] Chen D, Song D, Wientjes M G, et al. Clinical Cancer Research, 2003, 9: 363.
[11] Lu Z, Yeh T K, Tsai M, et al. Clinical Cancer Research, 2004, 10: 7677.
[12] LI Bu-hong, ZHANG Zhen-xi, XIE Shu-sen, et al(李步洪, 张镇西, 谢树森, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(7): 1310.
[13] HUANG Bao-hua, CHEN Rong, ZENG Hai-shan, et al(黄宝华, 陈荣, 曾海山, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(1): 95.
[14] WEI Hua-jiang, GUO Zhou-yi, XIE Shu-sen, et al(魏华江, 郭周义, 谢树森, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(9): 1757.
[15] WEI Hua-jiang, XING Da, HE Bo-hua, et al(魏华江, 邢达, 何博华, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(5): 868.
[16] FENG Shang-yuan, CHEN Rong, LI Yong-zeng, et al(冯尚源, 陈荣, 李永增, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(5): 712.
[17] WEI Hua-jiang, XING Da, WU Guo-yong, et al(魏华江, 邢达, 巫国勇, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(11): 1296.
[18] ZHU Dan, LUO Qing-ming, ZENG Shao-qun, et al(朱丹, 骆清铭, 曾绍群, 等). Acta Optica Sinica(光学学报), 2002, 22(3): 369.
[19] CHEN Rong, XIE Shu-sen, CHEN Yan-jiao, et al(陈荣, 谢树森, 陈艳娇, 等). J. Optoelectronics·Laser(光电子·激光), 2002, 13(1): 92.
[20] WANG L H. J. Opt. Soc. Am. A, 1998, 15(4): 936.
[21] Hammer M, Schweitzer D. Phys. Med. Biol., 2002, 47: 179.
[22] Parsa P, Jacques S L, Nishioka N S. Appl. Opt., 1989, 28(12): 2325.